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Open Access item Gene expression in developing zea mays embryos: regulation by Abscisic acid of a highly phosphorylated 23- to 25-kD group of proteins

Authors:Goday, Adela
Sánchez-Martínez, Demetrio
Gómez, Jordi
Puigdomènech, Pere
Pagès, Montserrat
Issue Date:Nov-1988
Publisher:American Society of Plant Biologists
Citation:Plant Physiology 88(3): 564-9 (1988)
Abstract:We have earlier identified a set of proteins of 23 to 25 kilodaltons (kD), covering an isoelectric point (pI) range of 6.2 to 8.2, which accumulate gradually during normal embryogenesis of Zea mays and disappear in early germination. These polypeptides can be induced prematurely in immature embryos by abscisic acid (ABA) treatment. We report here that the more acidic protein forms are due to post-translational phosphorylation of at least two polypeptides of 23 kD, pI 8.2 and 25 kD, pI 8.0. A polyclonal antiserum was obtained which recognizes all forms of both the 23-kD and 25-kD polypeptides. Recovery of cDNA clones corresponding to these proteins was accomplished by hybridization with cDNA made from size-selected mRNA enriched for these sequences. Hybrid selection experiments demonstrate that clone MA12 specifically hybridizes with mRNAs encoding the 23-kD and 25-kD protein set which are recognized by the antiserum. By Northern hybridization analysis, the RNA encoded by clone MA12 is shown to accumulate in mature embryos and to be induced in young embryos upon ABA incubation.
Description:6 pages.-- 7 figures.-- PMID: 16666349 [PubMed].-- PMCID: PMC1055625.
Publisher version (URL):http://www.plantphysiol.org/cgi/reprint/88/3/564
URI:http://hdl.handle.net/10261/26182
ISSN:0032-0889 (Print)
1532-2548 (Online)
Appears in Collections:(IQAC) Artículos

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