English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/26115
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Effect of UV-A and UV-B on diel patterns of growth and metabolic activity in Nannochloris atomus cultures assessed by flow cytometry

AuthorsSobrino, Cristina; Montero, Olimpio; Lubián, Luis M.
KeywordsUV radiation
Diel cycles
Flow cytometry
Nannochloris atomus
Issue DateJun-2005
PublisherInter Research
CitationMarine Ecology Progress Series 293: 29-35 (2005)
AbstractThe aim of this study was to assess the effect of UV-A (320 to 400 nm) and UV-B (280 to 320 nm) radiation on diel patterns of growth and metabolic activity of the marine picoplankter Nannochloris atomus using flow cytometry. N. atomus cells exposed to PAR (400 to 700 nm), PAR+UV-A and PAR+UV-A+UV-B showed clear diel patterns in cell size, chlorophyll fluorescence and metabolic activity, the latter being measured by a fluorescein diacetate-based cell esterase activity assay. For all spectral treatments, patterns increased during the day and decreased during the night, with minima near dawn and maxima near dusk. In addition, cell division was tightly phased to the light dark (L:D) cycle, occurring soon after dark. Exposure to UVR did not alter the synchrony of the parameters measured, but the extent of variation between dawn and dusk was dependent on the spectral conditions. Chlorophyll autofluorescence and metabolic activity decreased to a larger extent when cells were exposed to UV-B than in treatments where UV-B was excluded. In contrast, the cell size was larger under the treatment including UV-A+UV-B than under the treatment including only UV-A. These results show that UV-B damage can decrease growth and metabolic activity in N. atomus without altering the synchronization of the diel patterns, and contribute to a better understanding of phytoplankton behavior under UVR exposures.
Description7 pages, 2 figures, 2 tables.
Publisher version (URL)http://dx.doi.org/10.3354/meps293029
URIhttp://hdl.handle.net/10261/26115
DOI10.3354/meps293029
ISSN0171-8630
Appears in Collections:(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
m293p029.pdf109,02 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.