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dc.contributor.authorZapata, Manuel-
dc.contributor.authorEdvardsen, Bente-
dc.contributor.authorRodríguez, Francisco-
dc.contributor.authorMaestro, Miguel Ángel-
dc.contributor.authorGarrido, J. L.-
dc.identifier.citationMarine Ecology Progress Series 219: 85-98 (2001)en_US
dc.description14 pages, 6 figures, 6 tables.en_US
dc.description.abstractThe pigment composition of the haptophyte Chrysochromulina polylepis (Strain CCMP 286) was analysed by high-performance liquid chromatography (HPLC) using pyridine-containing mobile phases and polymeric C18, or monomeric C8 columns. The polar chlorophyll (chl) c pigment composition included chl c2 and chl c3 as major fractions, and divinyl protochlorophyllide a (DV pchlide or MgDVP) as a minor component. Several non-polar fluorescent peaks sharing a common chl c-type absorption spectrum were also detected. The main component of these peaks was isolated and characterised by chromatographic behaviour, UV-visible (UV-VIS) and fluorescence spectroscopy. Although spectral properties were similar to a high molecular weight non-polar chl c (1313 Da) recently characterised from Emiliania huxleyi, both chlorophylls showed different chromatographic behaviour. Fast atom bombardment-mass spectrometry (FAB-MS) analysis showed a high mass molecular ion (m/z 1265), and a fragmentation pattern compatible with a molecular structure consisting of a chl c2 pigment linked by an ester bond to the sugar moiety of a monogalactosyl diacylglyceride (MGDG), which included 2 myristic acid (14:0) residues. To study the distribution pattern of the novel pigment, different strains of C. polylepis and other species of the genus Chrysochromulina were analysed. A similar chl c2 ester was also present in 2 C. polylepis strains (K, B11) and in C. aff. polylepis (PLY 200), as well as in C. acantha, C. camella, C. leadbeateri, C. strobilus, C. throndsenii, and Chrysochromulina sp. (CS-410). Three species, C. fragaria, C. hirta (2 strains) and C. kappa, lacked the new chl c, but they contained detectable amounts of other non-polar chl c-like pigments. The feasibility of using chls c and carotenoids as marker pigments to detect Chrysochromulina species in field samples is discussed.en_US
dc.description.sponsorshipThis work was supported by funds from the Xunta de Galicia, Spain (PGDIDT-CIMA-99/9, PGDIDTMAR50102 projects).en_US
dc.format.extent487525 bytes-
dc.publisherInter Researchen_US
dc.subjectChlorophyll c2-galactolipid estersen_US
dc.subjectChrysochromulina polylepisen_US
dc.subjectChrysochromulina spp.en_US
dc.subjectFast atom bombardment-mass spectrometryen_US
dc.subjectHPLC pigment analysisen_US
dc.subjectMarker pigmentsen_US
dc.titleChlorophyll c2 monogalactosyldiacylglyceride ester (chl c2-MGDG). A novel marker pigment for Chrysochromulina species (Haptophyta)en_US
dc.description.peerreviewedPeer revieweden_US
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