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Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/25297
Title: Segregation of leading-edge and uropod components into specific lipid rafts during T cell polarization
Authors: Gómez-Moutón, Concepción; Abad, José Luis; Mira, Emilia; Lacalle, Rosa Ana; Gallardo, Eduard; Jiménez Baranda, Sonia; Illa, Isabel; Bernad, Antonio; Mañes, Santos; Martínez, Carlos
Keywords: Cell structure
T cell polarization
Leading-Edge Proteins
Issue Date: 14-Aug-2001
Publisher: National Academy of Sciences (U.S.)
Citation: Proceedings of the National Academy of Sciences of the USA 98(17): 9642-9647 (2001)
Abstract: Redistribution of specialized molecules in migrating cells develops asymmetry between two opposite cell poles, the leading edge and the uropod. We show that acquisition of a motile phenotype in T lymphocytes results in the asymmetric redistribution of ganglioside GM3- and GM1-enriched raft domains to the leading edge and to the uropod, respectively. This segregation to each cell pole parallels the specific redistribution of membrane proteins associated to each raft subfraction. Our data suggest that raft partitioning is a major determinant for protein redistribution in polarized T cells, as ectopic expression of raft-associated proteins results in their asymmetric redistribution, whereas non-raft-partitioned mutants of these proteins are distributed homogeneously in the polarized cell membrane. Both acquisition of a migratory phenotype and SDF-1α-induced chemotaxis are cholesterol depletion-sensitive. Finally, GM3 and GM1 raft redistribution requires an intact actin cytoskeleton, but is insensitive to microtubule disruption. We propose that membrane protein segregation not only between raft and nonraft domains but also between distinct raft subdomains may be an organizational principle that mediates redistribution of specialized molecules needed for T cell migration.
Description: 6 pages, 7 figures.-- PMID: 11493690 [PubMed].-- PMCID: PMC55505.-- Available online Aug 7, 2001.
Publisher version (URL): http://dx.doi.org/10.1073/pnas.171160298
URI: http://hdl.handle.net/10261/25297
ISSN: 0027-8424 (Print)
1091-6490 (Online)
DOI: 10.1073/pnas.171160298
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