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Properties of triple helices formed by parallel-stranded hairpins containing 8-aminopurines

AuthorsAviñó, Anna ; Frieden, Miriam; Morales, Juan Carlos; García de la Torre, Beatriz; Güimil García, Ramón; Azorín, Ferran ; Gelpí, José Luis; Orozco, Modesto; González, Carlos ; Eritja Casadellà, Ramón
Issue Date15-Jun-2002
PublisherOxford University Press
CitationNucleic Acids Research 30(12): 2609–2619 (2002)
AbstractParallel-stranded hairpins with a polypyrimidine sequence linked to a complementary purine carrying 8-aminopurines such as 8-aminoadenine, 8-aminoguanine and 8-aminohypoxanthine bind polypyrimidine sequences complementary (in an antiparallel sense) to the purine part by a triple helix. The relative stabilities of triplexes were assessed by UV-absorption melting experiments as a function of pH and salt concentration. Hairpins carrying 8-aminopurines give very stable triple helical structures even at neutral pH, as confirmed by gel-shift experiments, circular dichroism and nuclear magnetic resonance spectroscopy. The modified hairpins may be redesigned to cope with small interruptions in the polypyrimidine target sequence.
Description11 pages, 7 figures, 7 tables.-- PMID: 12060677 [PubMed].-- PMCID: PMC117286.-- Supporting information available at: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117286/bin/nar_30_12_2609__index.html
Publisher version (URL)http://www.ncbi.nlm.nih.gov/pmc/articles/PMC117286
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