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Open Access item Advances in methods for determining fecundity: application of the new methods to some marine fishes

Authors:Witthames, Peter R.
Thorsen, Anders
Murua, Hilario
Saborido-Rey, Fran
Greenwood, Lorraine N.
Domínguez-Petit, Rosario
Korta, María
Kjesbu, Olav S.
Issue Date:1-Apr-2009
Publisher:Scientific Publications Office
Citation:Fishery Bulletin 107(2): 148-164 (2009)
Abstract:Estimation of individual egg production (realized fecundity) is a key step either to understand the stock and recruit relationship or to carry out fisheries-independent assessment of spawning stock biomass using egg production methods. Many f ish are highly fecund and their ovaries may weigh over a kilogram; therefore the work time can be consuming and require large quantities of toxic fixative. Recently it has been shown for Atlantic cod (Gadus morhua) that image analysis can automate fecundity determination using a power equation that links follicles per gram ovary to the mean vitellogenic follicular diameter (the autodiametric method). In this article we demonstrate the precision of the autodiametric method applied to a range of species with different spawning strategies during maturation and spawning. A new method using a solid displacement pipette to remove quantitative fecundity samples (25, 50, 100, and 200 milligram [mg]) is evaluated, as are the underlying assumptions to effectively fix and subsample the ovary. Finally, we demonstrate the interpretation of dispersed formaldehyde-fixed ovarian samples (whole mounts) to assess the presence of atretic and postovulatory follicles to replace labor intensive histology. These results can be used to estimate down regulation (production of atretic follicles) of fecundity during maturation.
Description:17 pages, 9 figures, 6 tables.
Publisher version (URL):http://www.thefreelibrary.com/Advances+in+methods+for+determining+fecundity%3A+application+of+the+new+...-a0203602639
URI:http://hdl.handle.net/10261/25187
ISSN:0090-0656
Appears in Collections:(IIM) Artículos

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