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Advances in methods for determining fecundity: application of the new methods to some marine fishes

AuthorsWitthames, Peter R.; Thorsen, Anders; Murua, Hilario; Saborido-Rey, Fran ; Greenwood, Lorraine N.; Domínguez-Petit, Rosario ; Korta, María; Kjesbu, Olav S.
Issue Date1-Apr-2009
PublisherNational Marine Fisheries Service (U.S.)
CitationFishery Bulletin 107(2): 148-164 (2009)
AbstractEstimation of individual egg production (realized fecundity) is a key step either to understand the stock and recruit relationship or to carry out fisheries-independent assessment of spawning stock biomass using egg production methods. Many f ish are highly fecund and their ovaries may weigh over a kilogram; therefore the work time can be consuming and require large quantities of toxic fixative. Recently it has been shown for Atlantic cod (Gadus morhua) that image analysis can automate fecundity determination using a power equation that links follicles per gram ovary to the mean vitellogenic follicular diameter (the autodiametric method). In this article we demonstrate the precision of the autodiametric method applied to a range of species with different spawning strategies during maturation and spawning. A new method using a solid displacement pipette to remove quantitative fecundity samples (25, 50, 100, and 200 milligram [mg]) is evaluated, as are the underlying assumptions to effectively fix and subsample the ovary. Finally, we demonstrate the interpretation of dispersed formaldehyde-fixed ovarian samples (whole mounts) to assess the presence of atretic and postovulatory follicles to replace labor intensive histology. These results can be used to estimate down regulation (production of atretic follicles) of fecundity during maturation.
Description17 pages, 9 figures, 6 tables.
Publisher version (URL)http://www.thefreelibrary.com/Advances+in+methods+for+determining+fecundity%3A+application+of+the+new+...-a0203602639
Appears in Collections:(IIM) Artículos
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