Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/24910
Share/Export:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Title

The class I bHLH factors E2-2A and E2-2B regulate EMT

AuthorsSobrado, Verónica R.; Moreno-Bueno, Gema CSIC ORCID; Cubillo, Eva CSIC; Holt, Liam J.; Nieto, M. Ángela CSIC ORCID ; Portillo, Francisco CSIC; Cano, Amparo CSIC
KeywordsBhlh E2-2
E-cadherin repression
Emt
E47
Snail
Tumour progression
Gene profiling
Issue Date1-Apr-2009
PublisherCompany of Biologists
CitationJournal of Cell Science 122(7): 1014-1024 (2009)
AbstractFunctional loss of the cell-cell adhesion molecule E-cadherin is an essential event for epithelial-mesenchymal transition (EMT), a process that allows cell migration during embryonic development and tumour invasion. In most carcinomas, transcriptional repression has emerged as the main mechanism responsible for E-cadherin downregulation. Here, we report the identification of class I bHLH factor E2-2 (TCF4/ITF2) as a new EMT regulator. Both isoforms of E2-2 (E2-2A and E2-2B) induce a full EMT when overexpressed in MDCK cells but without affecting the tumorigenic properties of parental cells, in contrast to other EMT inducers, such as Snail1 or class I bHLH E47. E-cadherin repression mediated by E2-2 is indirect and independent of proximal E-boxes of the promoter. Knockdown studies indicate that E2-2 expression is dispensable for maintenance of the EMT driven by Snail1 and E47. Comparative gene-profiling analysis reveals that E2-2 factors induce similar, yet distinct, genetic programs to that induced by E47 in MDCK cells. These results, together with the embryonic expression pattern of Tcf4 and E2A (which encodes E12/E47), support a distinct role for E2-2 and suggest an interesting interplay between E-cadherin repressors in the regulation of physiological and pathological EMT processes.
Description11 pages, 6 figures.
Publisher version (URL)http://dx.doi.org/10.1242/jcs.028241
URIhttp://hdl.handle.net/10261/24910
DOI10.1242/jcs.028241
ISSN0021-9533
Appears in Collections:(IIBM) Artículos
(IN) Artículos




Files in This Item:
File Description SizeFormat
The class I bHLH.pdf2,89 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work

SCOPUSTM   
Citations

96
checked on May 24, 2022

WEB OF SCIENCETM
Citations

89
checked on May 25, 2022

Page view(s)

449
checked on May 29, 2022

Download(s)

327
checked on May 29, 2022

Google ScholarTM

Check

Altmetric

Dimensions


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.