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18F-choline images murine atherosclerotic plaques ex vivo

AuthorsMatter, Christian M.; Wyss, Matthias T.; Meier, Patricia; Lohmann, Christine; Weber, Bruno; Ramírez de Molina, Ana; Ametamey, Simon M.; Lacal, Juan Carlos ; Kaufmann, Philipp A.; Buck, Alfred
Apolipoprotein E knockout mice
Issue DateMar-2006
PublisherAmerican Heart Association
CitationArteriosclerosis Thrombosis and Vascular Biology 26(3): 584-589 (2006)
AbstractObjective— Current imaging modalities of atherosclerosis mainly visualize plaque morphology. Valuable insight into plaque biology was achieved by visualizing enhanced metabolism in plaque-derived macrophages using 18F-fluorodeoxyglucose (18F-FDG). Similarly, enhanced uptake of 18F-fluorocholine (18F-FCH) was associated with macrophages surrounding an abscess. As macrophages are important determinants of plaque vulnerability, we tested 18F-FCH for plaque imaging. Methods and Results— We injected 18F-FCH (n=5) or 18F-FDG (n=5) intravenously into atherosclerotic apolipoprotein E-deficient mice. En face measurements of aortae isolated 20 minutes after 18F-FCH injections demonstrated an excellent correlation between fat stainings and autoradiographies (r=0.842, P<0.0001), achieving a sensitivity of 84% to detect plaques by 18F-FCH. In contrast, radiotracer uptake 20 minutes after 18F-FDG injections correlated less with en face fat stainings (r=0.261, P<0.05), reaching a sensitivity of 64%. Histological analyses of cross-sections 20 minutes after coinjections of 18F-FCH and 14C-FDG (n=3) showed that 18F-FCH uptake correlated better with fat staining (r=0.740, P<0.0001) and macrophage-positive areas (r=0.740, P<0.0001) than 14C-FDG (fat: r=0.236, P=0.29 and CD68 staining: r=0.352, P=0.11), respectively. Conclusions— 18F-FCH identifies murine plaques better than 18F-FDG using ex vivo imaging. Enhanced 18F-FCH uptake into macrophages may render this tracer a promising candidate for imaging plaques in patients. For imaging plaque biology, we compared ex vivo autoradiographies and fat stainings of murine atherosclerotic aortae after injections of 18F-fluorocholine (18F-FCH) or 18F-fluorodeoxyglucose (18F-FDG). En face macroscopical and histological correlations were better for 18F-FCH as compared with 18F-FDG. Thus, 18F-FCH may be a promising compound for imaging plaques in patients.
Description15 pages, 3 figures, 1 table.-- et al.
Publisher version (URL)http://dx.doi.org/10.1161/01.ATV.0000200106.34016.18
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