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dc.contributor.authorHernández, Arturo-
dc.contributor.authorGarcía, Bibian-
dc.contributor.authorObregón, María Jesús-
dc.identifier.citationEndocrinology 148(8): 3968-3976 (2007)en_US
dc.description9 pages, 9 figures.en_US
dc.description.abstractActive thyroid hormones are critical for the differentiation and function of brown adipose tissue. However, we have observed high basal and induced levels of type 3 deiodinase (D3), an enzyme that inactivates thyroid hormones and is coded by the imprinted gene Dio3, in differentiating brown preadipocytes in primary culture. We find that D3 activity and mRNA expression strongly correlate with the rate of proliferation of undifferentiated precursor cells under various conditions. Furthermore, differentiation of precursor cells to adipocytes is associated with decreased levels of D3 expression, and only very low levels of D3 mRNA are found in mature adipocytes. Dlk1, an inhibitor of adipocyte differentiation and a paternally expressed gene located in the same imprinted domain as Dio3, displayed changes in expression that parallel those of Dio3. In contrast, a 4-kb transcript for Dio3os, an antisense gene also located in the same imprinted domain, is markedly up-regulated in differentiated adipocytes. We conclude that D3 expression in differentiating preadipocytes is primarily linked to proliferating cells, whereas Dio3os expression is associated with mature adipocytes. Our results suggest that genomic imprinting and gene expression at the Dlk1/Dio3 imprinted domain may play a role in the regulation of adipocyte proliferation and differentiation.en_US
dc.description.sponsorshipThis work was supported by Research Grants PB 95-0097 from Direction General de Investigacion Cientifica y Tecnica (DGICYT) and 94/0274 from Fondo de Investigaciones Sanitarias (FISS) (Spain), Plan Nacional SAF2006-01319, and National Institutes of Health Grant DK054716 from the National Institute of Diabetes and Digestive and Kidney Diseases.en_US
dc.format.extent467970 bytes-
dc.publisherEndocrine Societyen_US
dc.titleGene expression from the imprinted Dio3 locus is associated with cell proliferation of cultured brown adipocytesen_US
dc.description.peerreviewedPeer revieweden_US
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