Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/24644
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | A permissive retinoid X receptor/thyroid hormone receptor heterodimer allows stimulation of prolactin gene transcription by thyroid hormone and 9-cis-retinoic acid |
Autor: | Castillo, Ana I. CSIC ORCID; Sánchez-Martínez, Ruth CSIC ORCID; Moreno, José L. CSIC; Martínez-Iglesias, Olaia CSIC; Palacios, Daniela; Aranda, Ana CSIC ORCID | Fecha de publicación: | ene-2004 | Editor: | American Society for Microbiology | Citación: | Molecular and Cellular Biology 24(2): 502-513 (2004) | Resumen: | Heterodimers of the retinoid X receptor (RXR) with the thyroid hormone receptor (TR) are considered to be nonpermissive. It is believed that within these complexes RXR acts as a "silent partner." We demonstrate here that a permissive heterodimer mediates stimulation of prolactin expression by the thyroid hormone T3 and by 9-cis retinoic acid (9-cis-RA). A response element located in the prolactin distal enhancer mediates transactivation by both ligands in pituitary cells, and RXR recruits coactivators when bound to this element as a heterodimer with TR. Furthermore, transcription by the RXR agonist can be obtained in CV-1 cells only after overexpression of coactivators, and overexpression of corepressors inhibits the response in pituitary cells. Thus, cell type-specific differences in coregulator recruitment can determine the cellular response to both ligands. Coactivator recruitment by 9-cis-RA requires the ligand-dependent transactivation domains (AF-2) of both heterodimeric partners. Interestingly, the presence of the RXR ligand can overcome the deleterious effect of the AF-2 mutation E401Q on association with coactivators and transactivation. These results demonstrate an unexpected role for RXR in TR signaling and show that in particular cellular environments this receptor can act as a "nonsilent" partner of TR, allowing stimulation by RXR agonists. | Descripción: | 12 pages, 10 figures. | Versión del editor: | http://dx.doi.org/10.1128/MCB.24.2.502-513.2004 | URI: | http://hdl.handle.net/10261/24644 | DOI: | 10.1128/MCB.24.2.502-513.2004 | ISSN: | 0270-7306 |
Aparece en las colecciones: | (IIBM) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
accesoRestringido.pdf | 15,38 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
29
checked on 16-abr-2024
SCOPUSTM
Citations
67
checked on 16-abr-2024
WEB OF SCIENCETM
Citations
59
checked on 29-feb-2024
Page view(s)
384
checked on 19-abr-2024
Download(s)
99
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.