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Closed Access item Role of HuR in skeletal myogenesis through coordinate regulation of muscle differentiation genes

Authors:Figueroa, Angélica
Cuadrado, Ana
Gorospe, Myriam
Muñoz Terol, Alberto
Issue Date:Jul-2003
Publisher:American Society for Microbiology
Citation:Molecular and Cellular Biology 23(14): 4991-5004 (2003)
Abstract:In this report, we investigate the role of the RNA-binding protein HuR during skeletal myogenesis. At the onset of myogenesis in differentiating C2C12 myocytes and in vivo in regenerating mouse muscle, HuR cytoplasmic abundance increased dramatically, returning to a predominantly nuclear presence upon completion of myogenesis. mRNAs encoding key regulators of myogenesis-specific transcription (myogenin and MyoD) and cell cycle withdrawal (p21), bearing AU-rich regions, were found to be targets of HuR in a differentiation-dependent manner. Accordingly, mRNA half-lives were highest during differentiation, declining when differentiation was completed. Importantly, HuR-overexpressing C2C12 cells displayed increased target mRNA expression and half-life and underwent precocious differentiation. Our findings underscore a critical function for HuR during skeletal myogenesis linked to HuR's coordinate regulation of muscle differentiation genes.
Description:14 pages, 7 figures.-- et al.
Publisher version (URL):http://dx.doi.org/10.1128/MCB.23.14.4991-5004.2003
Appears in Collections:(IIBM) Artículos

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