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Open Access item Crystallization and preliminary X-ray diffraction analysis of the fructofuranosidase from Schwanniomyces occidentalis

Authors:Polo, Aitana
Alvaro-Benito, Miguel
Fernández-Lobato, María
Sanz-Aparicio, J.
Keywords:Schwanniomyces occidentalis, X-ray crystallographic analyses
Issue Date:2010
Publisher:Blackwell Publishing
Citation:Acta Cryst. (2009). F65, 1162–1165
Abstract:Schwanniomyces occidentalis invertase is an extracellular enzyme that releases -fructose from the nonreducing termini of various -d-fructofuranoside substrates. Its ability to produce 6-kestose by transglycosylation makes this enzyme an interesting research target for applications in industrial biotechnology. The enzyme has been expressed in Saccharomyces cerevisiae. Recombinant and wildtype forms, which showed different glycosylation patterns, were crystallized by vapour-diffusion methods. Although crystallization trials were conducted on both forms of the protein, crystals suitable for X-ray crystallographic analyses were only obtained from the wild-type enzyme. The crystals belonged to space group P212121, with unit-cell parameters a = 105.78, b = 119.49, c = 137.68 A ° . A diffraction data set was collected using a synchrotron source. Self-rotation function and sedimentation-velocity experiments suggested that the enzyme was dimeric with twofold symmetry.
Publisher version (URL):http://dx.doi.org/10.1107/S1744309109039384
URI:http://hdl.handle.net/10261/24450
ISSN:1744-3091
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