Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism

Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/24437

COMPARTIR / EXPORTAR:

SHARE BASE	Comparte tu historia de Acceso Abierto
Visualizar otros formatos: MARC \| Dublin Core \| RDF \| ORE \| MODS \| METS \| DIDL \| DATACITE
Refman EndNote Bibtex RefWorks Excel CSV PDF DataCite Send via email

Campo DC	Valor	Lengua/Idioma
dc.contributor.author	Vincent, Olivier	-
dc.contributor.author	Carlson, Marian	-
dc.date.accessioned	2010-05-18T12:07:29Z	-
dc.date.available	2010-05-18T12:07:29Z	-
dc.date.issued	2001-05-01	-
dc.identifier.citation	Genes and Development 15(9): 1104-1114 (2001)	en_US
dc.identifier.issn	0890-9369	-
dc.identifier.uri	http://hdl.handle.net/10261/24437	-
dc.description	12 pages, 8 figures, 1 table.-- et al.	en_US
dc.description.abstract	The Snf1/AMP-activated protein kinase family has broad roles in transcriptional, metabolic, and developmental regulation in response to stress. In Saccharomyces cerevisiae, Snf1 is required for the response to glucose limitation. Snf1 kinase complexes contain the alpha (catalytic) subunit Snf1, one of the three related beta subunits Gal83, Sip1, or Sip2, and the gamma subunit Snf4. We present evidence that the beta subunits regulate the subcellular localization of the Snf1 kinase. Green fluorescent protein fusions to Gal83, Sip1, and Sip2 show different patterns of localization to the nucleus, vacuole, and/or cytoplasm. We show that Gal83 directs Snf1 to the nucleus in a glucose-regulated manner. We further identify a novel signaling pathway that controls this nuclear localization in response to glucose phosphorylation. This pathway is distinct from the glucose signaling pathway that inhibits Snf1 kinase activity and responds not only to glucose but also to galactose and sucrose. Such independent regulation of the localization and the activity of the Snf1 kinase, combined with the distinct localization of kinases containing different beta subunits, affords versatility in regulating physiological responses.	en_US
dc.description.sponsorship	This work was supported by NIH grant GM34095 to M.C. R.T. also received support from NIH training grant T32 GM08224.	en_US
dc.format.extent	723253 bytes	-
dc.format.mimetype	application/pdf	-
dc.language.iso	eng	en_US
dc.publisher	Cold Spring Harbor Laboratory Press	en_US
dc.rights	closedAccess	en_US
dc.subject	Snf1/AMPK kinases	en_US
dc.subject	Yeast	en_US
dc.subject	Nuclear localization	en_US
dc.subject	Glucose signaling	en_US
dc.title	Subcellular localization of the Snf1 kinase is regulated by specific beta subunits and a novel glucose signaling mechanism	en_US
dc.type	artículo	en_US
dc.description.peerreviewed	Peer reviewed	en_US
dc.relation.publisherversion	http://genesdev.cshlp.org/content/15/9/1104.long	en_US
dc.type.coar	http://purl.org/coar/resource_type/c_6501	es_ES
item.openairetype	artículo	-
item.grantfulltext	none	-
item.cerifentitytype	Publications	-
item.openairecristype	http://purl.org/coar/resource_type/c_18cf	-
item.fulltext	No Fulltext	-
item.languageiso639-1	en	-
Aparece en las colecciones:	(IIBM) Artículos