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dc.contributor.authorCamarero, Guadalupe-
dc.contributor.authorAvendaño, Carlos-
dc.contributor.authorFernández-Moreno, Carmen-
dc.contributor.authorVillar, Ángeles-
dc.contributor.authorContreras, Julio-
dc.contributor.authorPablo, Flora de-
dc.contributor.authorPichel, José G.-
dc.contributor.authorVarela-Nieto, Isabel-
dc.identifier.citationJournal of Neuroscience 21(19): 7630-7641 (2001)en_US
dc.description12 pages, 7 figures, 2 tables.en_US
dc.description.abstractInsulin-like growth factor-1 (IGF-1) has been shown to play a key role during embryonic and postnatal development of the CNS, but its effect on a sensory organ has not been studied in vivo. Therefore, we examined cochlear growth, differentiation, and maturation in Igf-1 gene knock-out mice at postnatal days 5 (P5), P8, and P20 by using stereological methods and immunohistochemistry. Mutant mice showed reduction in size of the cochlea and cochlear ganglion. An immature tectorial membrane and a significant decrease in the number and size of auditory neurons were also evident at P20. IGF-1-deficient cochlear neurons showed increased caspase-3-mediated apoptosis, along with aberrant expression of the early neural markers nestin and Islet 1/2. Cochlear ganglion and fibers innervating the sensory cells of the organ of Corti presented decreased levels of neurofilament and myelin P(0) in P20 mouse mutants. In addition, an abnormal synaptophysin expression in the somata of cochlear ganglion neurons and sensory hair cells suggested the persistence of an immature pattern of synapses distribution in the organ of Corti of these animals. These results demonstrate that lack of IGF-1 in mice severely affects postnatal survival, differentiation, and maturation of the cochlear ganglion cells and causes abnormal innervation of the sensory cells in the organ of Corti.en_US
dc.description.sponsorshipThis work was supported by Dirección General de Investigación y Desarrollo (Spain) Grants PM96.0075 and PM99–0111 (I.V.-N.) and PM97.0143 (F.d.P.) and Autonomous Community of Madrid grant CAM 08.5/0023/98 (C.A.). The fellowships to G.C. and C.F.-M. were awarded by the Ministerio de Educación y Cultura (Spain).en_US
dc.format.extent1967365 bytes-
dc.publisherSociety for Neuroscienceen_US
dc.subjectCochlear ganglionen_US
dc.subjectInsulin-like growth factor 1en_US
dc.subjectOrgan of Cortien_US
dc.titleDelayed inner ear maturation and neuronal loss in postnatal Igf-1-deficient miceen_US
dc.description.peerreviewedPeer revieweden_US
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