Please use this identifier to cite or link to this item:
logo share SHARE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Triiodothyronine amplifies the adrenergic stimulation of uncoupling protein expression in rat brown adipocytes

AuthorsHernández, Arturo; Obregón, María Jesús
KeywordsThyroid hormones
Brown adipose tissue
Issue DateMay-2000
PublisherAmerican Physiological Society
CitationAJP - Endocrinology and Metabolism 278(5): E769-E777 (2000)
AbstractUncoupling protein (UCP), the mitochondrial protein specific to brown adipose tissue, is activated transcriptionally in response to cold and adrenergic agents. We studied the role of triiodothyronine (T(3)) on the adrenergic stimulation of UCP mRNA expression by use of primary cultures of rat brown adipocytes. Basal UCP mRNA levels are undetectable. Norepinephrine (NE) increases UCP mRNA during differentiation, not during proliferation. In hypothyroid conditions, UCP mRNA response to NE is almost absent. The presence of T(3) (0.2-20 nM) greatly increases the adrenergic response (30-fold). The sensitivity of UCP mRNA responses to NE is potentiated approximately 100-fold by the presence of T(3). The effect is proportional to the dose and time of preexposure to T(3). The increases obtained with NE and T(3) are prevented by actinomycin and cycloheximide. T(3) greatly stabilizes UCP mRNA transcripts. The effects of thyroxine and retinoic acid are weaker than those of T(3). In conclusion, in cultured rat brown adipocytes, T(3) is required and both synergizes with NE to increase UCP mRNA and stabilizes its mRNA transcripts.
Description9 pages, 9 figures.-- A page with additional content.
Publisher version (URL)
Appears in Collections:(IIBM) Artículos

Files in This Item:
File Description SizeFormat
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
Show full item record
Review this work

Page view(s)

checked on May 23, 2022


checked on May 23, 2022

Google ScholarTM


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.