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Open Access item RNA nuclear export is blocked by poliovirus 2A protease and is concomitant with nucleoporin cleavage
Izquierdo, José M.
|Keywords:||2A protease, RNA nuclear export, Nucleoporin, Interferon, Gene Expression Control|
|Publisher:||Company of Biologists|
|Citation:||Journal of Cell Science 122, 3799-3809 (2009)|
|Abstract:||Cytopathic viruses have developed successful strategies to block
or, at least, to attenuate host interference with their replication.
Here, we have analyzed the effects of poliovirus 2A protease on
RNA nuclear export. 2A protease interferes with trafficking of
mRNAs, rRNAs and U snRNAs from the nucleus to the
cytoplasm, without any apparent effect on tRNA transport.
Traffic of newly produced mRNAs is more strongly affected than
traffic of other mRNAs over-represented in the cytoplasm, such
as mRNA encoding -actin. Inhibition of RNA nuclear export
in HeLa cells expressing 2A protease is concomitant with the
cleavage of Nup98, Nup153, Nup62 and their subsequent
subcellular redistribution. The expression of an inactive 2A
protease failed to interfere with RNA nuclear export. In
addition, other related proteases, such as poliovirus 3C or foot
and mouth disease virus Lpro did not affect mRNA distribution
or Nup98 integrity. Treatment of HeLa cells with interferon
(IFN)- increased the relative amount of Nup98. Under such
conditions, the cleavage of Nup98 induced by 2A protease is
partial, and thus IFN- prevents the inhibition of RNA nuclear
export. Taken together, these results are consistent with a
specific proteolysis of Nup98 by 2A protease to prevent de novo
mRNA traffic in poliovirus-infected cells.|
|Publisher version (URL):||http://dx.doi.org/doi:10.1242/jcs.055988|
|Appears in Collections:||(CBM) Artículos|
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