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Open Access item An easy procedure to transform the ratio of two polynomials of first degree into Michaelis-Menten-type equations. Application to the ordered Uni Bi enzyme mechanism

Authors:Fontes, Rui
Meireles Ribeiro, João
Sillero, Antonio
Keywords:Enzyme equations, Enzyme inhibition, Enzyme kinetics, Graphical presentations, Rectangular hyperbolas
Issue Date:2000
Publisher:Panstwowe Wydawnictwo Naukowe
Citation:Acta Biochimica Polonica 47(1): 259-268 (2000)
Abstract:It is not always clear that some equations affected by complicated factors can, actually, be interpreted as a ratio of two polynomials of first degree and so that they can be, in general, represented by rectangular hyperbolas. In this paper we present an easy procedure to rearrange those equations into Michaelis-Menten-type equations and so to make the aspects of these rectangular hyperbolas more clear, particularly for researchers familiar with general biochemistry. As an example, the method is applied to transform the classical rate equation of the Cleland's Ordered Uni Bi enzyme mechanism.
Description:10 pages, 2 figures, 27 equations.
Publisher version (URL):http://www.actabp.pl/#File?./html/1_2000/259.html
URI:http://hdl.handle.net/10261/24185
ISSN:0001-527X
Appears in Collections:(IIBM) Artículos

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