Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/23845
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Title

Regulation of malic-enzymes-gene expression by cAMP and retinoic acid in differentiating brown adipocytes

AuthorsHernández, Arturo; García-Jiménez, Custodia; Santisteban, Pilar CSIC ORCID; Obregón, María Jesús
Issue DateJul-1993
PublisherWiley-Blackwell
CitationEuropean Journal of Biochemistry 215(2): 285-290 (1993)
AbstractBrown adipose tissue (BAT) is composed of highly specialized cells, whose main function is to produce heat under adrenergic stimulation, uncoupling oxidative phosphorylation. For this function, lipogenesis must be accurately regulated. Malic enzyme has a central role in lipogenesis and is strongly expressed in brown adipocytes. In this work, we study the modulation by adrenergic stimuli, cAMP effectors and retinoic acid on the induction produced by insulin and 3,5,3'-triiodothyronine on malic-enzyme-gene expression. Primary cultures of differentiating brown adipocytes have been used. The results obtained demonstrate that physiological doses of norepinephrine do not modify malic-enzyme mRNA levels when acting alone, but considerably reduce the induction produced by insulin, 3,5,3'-triiodothyronine or both together. Other cAMP inducers such as glucagon, forskolin or 8-bromo-cAMP, greatly inhibit both, basal and 3,5,3'-triiodothyronine-induced malic-enzyme-gene expression. Retinoic acid abolishes basal and also inhibits 3,5,3'-triiodothyronine-induced malic-enzyme-gene expression.
Description6 pages, 5 figures.-- Now known as FEBS Journal: Open Access content older than 1 year.
Publisher version (URL)http://dx.doi.org/10.1111/j.1432-1033.1993.tb18033.x
URIhttp://hdl.handle.net/10261/23845
DOI10.1111/j.1432-1033.1993.tb18033.x
ISSN0014-2956
Appears in Collections:(IIBM) Artículos




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