| Título: | Genetic tool development in marine protists: emerging model organisms for experimental cell biology |
Autor: | Faktorová, Drahomíra; Nisbet, R. Ellen R; Robledo, José A. F. CSIC; Casacuberta, Elena CSIC ORCID ; Sudek, Lisa; Allen, Andrew E.; Ares, Manuel; Aresté, Cristina CSIC; Balestreri, Cecilia; Barbrook, Adrian C.; Beardslee, Patrick; Ruaud, Albane; Ruiz-Trillo, Iñaki CSIC ORCID ; Sigg, Monika A.; Silver, Pamela A.; Slamovits, Claudio H.; Smith, G. Jason; Sprecher, Brittany N.; Stern, Rowena; Swart, Estienne C.; Tsaousis, Anastasios D.; Valach, Matus; Tsypin, Lev; Turkewitz, Aaron; Turnšek, Jernej; Vergé, Valérie; von Dassow, Peter; von der Haar, Tobias; Waller, Ross F.; Wang, Lu; Wen, Xiaoxue; Wheeler, Glen; Woods, April; Zhang, Huan; Bender, Sara; Mock, Thomas; Worden, Alexandra Z.; Lukeš, Julius; Booth, David S.; Bouget, François-Yves; Bowler, Chris; Breglia, Susana A.; Brownlee, Colin; Burger, Gertraud; Cerutti, Heriberto; Cesaroni, Rachele; Chiurillo, Miguel A.; Clemente, Thomas; Coles, Duncan B.; Collier, Jackie L.; Cooney, Elizabeth C.; Coyne, Kathryn; Docampo, Roberto; Dupont, Christopher L.; Edgcomb, Virginia; Einarsson, Elin; Elustondo, Pía A.; Federici, Fernan; Freire-Beneitez, Verónica; Freyria, Nastasia J.; Fukuda, Kodai; García, Paulo A.; Girguis, Peter R.; Gomaa, Fatma; Gornik, Sebastian G.; Guo, Jian; Hampl, Vladimir; Hanawa, Yutaka; Haro-Contreras, Esteban R.; Hehenberger, Elisabeth; Highfield, Andrea; Hirakawa, Yoshihisa; Hopes, Amanda; Howe, Christopher J.; Hu, Ian; Ibañez, Jorge; Irwin, Nicholas A. T.; Ishii, Yuu; Janowicz, Natalia Ewa; Jones, Adam C.; Kachale, Ambar; Fujimura-Kamada, Konomi; Kaur, Binnypreet; Kaye, Jonathan Z.; Kazana, Eleanna; Keeling, Patrick J.; King, Nicole; Klobutcher, Lawrence A.; Lander, Noelia; Lassadi, Imen; Li, Zhuhong; Lin, Senjie; Lozano, Jean-Claude; Luan, Fulei; Maruyama, Shinichiro; Matute, Tamara; Miceli, Cristina; Minagawa, Jun; Moosburner, Mark; Najle, Sebastián R. CSIC ORCID; Nanjappa, Deepak; Nimmo, Isabel C.; Noble, Luke; Novák Vanclová, Anna M. G.; Nowacki, Mariusz; Núñez, Isaac; Pain, Arnab; Piersanti, Angela; Pucciarelli, Sandra; Pyrih, Jan; Rest, Joshua S.; Rius, Mariana; Robertson, Deborah |
Palabras clave: | Genetic models
Molecular biology |
Fecha de publicación: | 6-abr-2020 |
Editor: | Nature Publishing Group |
Citación: | Nature Methods 17: 481-494 (2020) |
Resumen: | Diverse microbial ecosystems underpin life in the sea. Among these microbes are many unicellular eukaryotes that span the diversity of the eukaryotic tree of life. However, genetic tractability has been limited to a few species, which do not represent eukaryotic diversity or environmentally relevant taxa. Here, we report on the development of genetic tools in a range of protists primarily from marine environments. We present evidence for foreign DNA delivery and expression in 13 species never before transformed and for advancement of tools for eight other species, as well as potential reasons for why transformation of yet another 17 species tested was not achieved. Our resource in genetic manipulation will provide insights into the ancestral eukaryotic lifeforms, general eukaryote cell biology, protein diversification and the evolution of cellular pathways. |
Versión del editor: | http://dx.doi.org/10.1038/s41592-020-0796-x |
URI: | http://hdl.handle.net/10261/236663 |
DOI: | 10.1038/s41592-020-0796-x |
Identificadores: | doi: 10.1038/s41592-020-0796-x
e-issn: 1548-7105
issn: 1548-7091 |
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