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Detection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR

AuthorsMercado-Blanco, Jesús ; Rodríguez-Jurado, Dolores; Pérez-Artés, Encarnación ; Jiménez-Díaz, Rafael M.
KeywordsMolecular diagnosis
Olea europaea
Free certification programs
Verticillium wilt
Issue DateJan-2002
CitationEuropean Journal of Plant Pathology (2002), 108: 1–13
AbstractSpread of Verticillium wilt into newly established olive orchards in Andaluc´ıa, southern Spain, has caused concern in the olive industry in the region. This spread may result from use of Verticillium dahliae-infected planting material, which can extend distribution of the highly virulent, defoliating (D) pathotype of V. dahliae to newareas. In this study, a molecular diagnostic method for the early in planta detection of D V. dahliae was developed, aimed especially at nursery-produced olive plants. For this purpose, new primers for nested PCR were designed by sequencing a 992-bp RAPDmarker of theDpathotype. The use of the specific primers and different nested-PCR protocols allowed the detection of V. dahliae pathotype D DNA in infected root and stem tissues of young olive plants. Detection of the pathogen was effective from the very earliest moments following inoculation of olive plants with a V. dahliae pathotype D conidia suspension as well as in inoculated, though symptomless, plants.
Description13 pages, 6 figures
Publisher version (URL)http://dx.doi.org/10.1023/A:1013994827836
Appears in Collections:(IAS) Artículos
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