English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/23273
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Correlation of molecular markers and biological properties in Verticillium dahliae and the possible origins of some isolates

AuthorsCollins, A.; Mercado-Blanco, Jesús ; Jiménez-Díaz, Rafael M. ; Olivares, C.; Clewes, E.; Barbara, D. J.
Interspecific hybrids
Vegetative compatibility
Wilt diseases
Issue DateAug-2005
PublisherBritish Society for Plant Pathology
CitationPlant Pathology (2005), 54: 549–557
AbstractHaploid and amphihaploid Verticillium dahliae isolates were studied using PCR-based molecular markers which: (i) discriminate the defoliating and nondefoliating pathotypes (two primer pairs INTD2f/ r and INTND2f/ r), and (ii) are speciesspecific (primer pair 19/22). The results were compared with some known biological and other molecular properties of the isolates. Five discrete sequences of the 19/22 amplicon were found. Sequence 4 was associated with both defoliating isolates from Spain and nondefoliating isolates from Spain and USA; these pathotypes were separated by the primer pairs INTD2f/ r and INTND2f/ r, but the data showed that the primer espdef01 (derived from the 19/22 amplicon) cannot be used for this purpose. Amplicon sizes and sequences with primers 19/22 divided amphihaploid isolates from crucifers (thought to be interspecific hybrids) into those corresponding to the previously reported α and β groups. The β -group isolates had either sequence 4 or 5 (these two differing by a single base). The distinct amplicon sequence 3 given by the α -group isolates demonstrated that the V. dahliae -like ‘parent’ of this group was molecularly unlike any haploid isolate yet studied. The overall results are discussed in relation to phytosanitary considerations and the probability of defoliating or crucifer pathotypes arising de novo within Europe, either by selection or by interspecific hybridizations.
Description9 pages; 3 tables; 1 figure
Publisher version (URL)http://dx.doi.org/10.1111/j.1365-3059.2005.01240.x
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
Digital CSIC. Carta.pdf153,65 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.