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Título: | Efficient and Low Cytotoxicity Gene Carriers Based on Amine-Functionalized Polyvinylpyrrolidone |
Autor: | Prado, Anselmo del CSIC ORCID; Civantos, Ana CSIC; Martínez-Campos, Enrique CSIC ORCID; Levkin, P.; Reinecke, Helmut CSIC ORCID; Gallardo Ruiz, Alberto CSIC ORCID; Elvira, Carlos CSIC ORCID | Palabras clave: | Polyvinylpyrrolidone Cationic polymers Biomaterials Cytocompatibility Transfection |
Fecha de publicación: | 17-nov-2020 | Editor: | Multidisciplinary Digital Publishing Institute | Citación: | Polymers 12(11): 2724 (2020) | Resumen: | Non-viral vectors are a safety tool for gene therapy to deliver therapeutic genes. Among the different non-viral vectors, polyvinylpyrrolidone (PVP), a well-known hydrosoluble, neutral, and non-toxic polymer, satisfies the requirements and becomes a suitable candidate for gene delivery. In this study, we describe the preparation of polyvinylpyrrolidones decorated with pyrrolidine, piperidine, and piperazine groups, and evaluate them in vitro as non-viral gene carriers. The properties of these new systems are compared with those of hyperbranched polyethyleneimine (PEI) used as a positive control. Their ability to complex DNA at different N/P molar ratios, from 1:1 up to 10:1, was studied through agarose gel electrophoresis and dynamic light scattering. The resulting complexes (polyplexes) were characterized and evaluated in vitro with murine fibroblast (Swiss 3T3) as non-viral gene carriers, using luciferase as the reporter gene and a calcein cytocompatibility assay. All the copolymers condensed DNA to a particle average size between 100¿400 nm when used at N/P ratios of 4:1 or higher. The copolymers with piperidine groups showed higher transfection efficiency than the pyrrolidine and piperazine modified copolymers, and even higher than the positive control of PEI at N/P ratios of 4:1 or higher. All the synthesized polyplexes from an aminated PVP displayed a general tendency of high cytocompatibility (75¿95%) in comparison with the positive control PEI (55%). | Versión del editor: | http://dx.doi.org/10.3390/polym12112724 | URI: | http://hdl.handle.net/10261/229434 | DOI: | 10.3390/polym12112724 | Identificadores: | doi: 10.3390/polym12112724 issn: 2073-4360 |
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Polyvinylpyrrolidone.pdf | 3,28 MB | Adobe PDF | Visualizar/Abrir |
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