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Isolation and characterization of a mutant of Saccharomyces cerevisiae defective in phosphoenolpyruvate carboxykinase

AuthorsPerea, Javier; Gancedo, Carlos
KeywordsPhosphoenolpyruvate carboxykinase
saccharomyces cerevisiae
Issue Date1982
CitationArchives of Microbiology 132(2): 141-143 (1982)
AbstractA mutant of Saccharomices cerevisiae lacking phosphoenolpyruvate carboxykinase (E.C. was isolated. The mutant did not grow on gluconeogenic sources except glycerol. The mutation was recessive and apparently affected the structural gene of the enzyme. Intracellular levels of metabolites related to the metabolic situation of the enzyme were not significantly affected after trasfer of the mutant from a medium with glycerol to a medium with ethanol as carbon source. In these conditions only AMP decreased 3 to 5 times. A search for mutants affected in the other gluconeogenic enzyme, fructose 1,6 biphosphatase, remained unsuccessful.
DescriptionPubmed PMID: 6751258
Publisher version (URL)http://link.springer.com/article/10.1007%2FBF00508719#
Appears in Collections:(IIBM) Artículos
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