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dc.contributor.authorJauzein, Cécile-
dc.contributor.authorCollos, Yves-
dc.contributor.authorGarcés, Esther-
dc.contributor.authorVila, Magda-
dc.contributor.authorMasó, Mercedes-
dc.date.accessioned2010-03-19T09:27:27Z-
dc.date.available2010-03-19T09:27:27Z-
dc.date.issued2008-10-
dc.identifier.citationJournal of Phycology 44(5): 1136-1145 (2008)en_US
dc.identifier.issn0022-3646-
dc.identifier.urihttp://hdl.handle.net/10261/22532-
dc.description10 pages, 4 figures, 3 tablesen_US
dc.description.abstractIn batch cultures of four Mediterranean strains (from France, Italy, and Spain) of Alexandrium catenella (Whedon et Kof.) Balech growing on a daily light cycle, ammonium and urea uptake were estimated by the 15N tracer technique. Ammonium uptake could be described by Michaelis-Menten kinetics along a substrate gradient of 0.1-10 μgat N · L−1 for the four strains, while two different patterns were observed for urea uptake with Michaelis-Menten kinetics for one strain and linear kinetics for the others. In all cases, an increase in uptake rates with time was noted over the daylight period. This trend led to a net increase in the maximum uptake rate (Vmax; for saturable kinetics) and in the initial slope α. For ammonium, Vmax increased by a factor of 2-10 depending on the strain, and, for urea, the maximal uptake rates measured increased by a factor of 2-18. Temporal variations of half-saturation constants (Ks) for both nutrients did not show a clear trend. Increases in Vmax and α showed an acclimation of the cells' uptake system over time to a N pulse, which may be explained by the light periodicity. For two strains, extensive ammonium release was observed during urea assimilation. This mechanism removes urea from the medium, so it is no longer available to other potential competitors, but supplies N back to the medium in the form of ammonium. From a methodological point of view, the phenomenon leads to considerable underestimates of the contribution of urea to phytoplankton growthen_US
dc.description.sponsorshipThe authors wish to thank R. Ventosa for the nutrient analyses. We thank S. Fraga for the strain ACATA4. This study was financed by the CNRS (Centre National de la Recherche cientifique) and Ifremer (Institut Français pour l’Exploitation de la Mer), as well as the EU-funded Research Project SEED (Life cycle transformations among HAB species, and the environmental and physiological factors that regulate them, GOCE-CT-2005-003875). E. G.’s work was sustained by a Ramon y Cajal contract from the Spanish Ministry of Science and Education. This work benefited from correspondence with Dr. T. A. V. Reesen_US
dc.format.extent22195 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherPhycological Society of Americaen_US
dc.rightsclosedAccessen_US
dc.subjectAlexandrium catenellaen_US
dc.subjectAmmonium-
dc.subjectAmmonium release-
dc.subjectUptake kinetics-
dc.subjectUrea-
dc.subjectTemporal availability-
dc.titleShort-term temporal variability of ammonium and urea uptake by Alexandrium Catenella (Dinophyta) in culturesen_US
dc.typeartículoen_US
dc.identifier.doi10.1111/j.1529-8817.2008.00570.x-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://doi.org/10.1111/j.1529-8817.2008.00570.xen_US
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.languageiso639-1en-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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