English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/21844
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

The use of flow cytometry for species identification and life-cycle studies in dinoflagellates

AuthorsFigueroa, Rosa Isabel ; Garcés, Esther ; Bravo, Isabel
KeywordsAlexandrium
DNA content
Flow cytometry
Karlodinium
Sexuality
Issue DateFeb-2010
PublisherElsevier
CitationDeep Sea Research - Part II - Topical Studies in Oceanography 57(3-4): 301-307 (2010)
AbstractThe difficulties encountered in attempts to differentiate between dinoflagellate species of the genera Alexandrium and Karlodinium using morphological characteristics are well-known. For this reason, species of these genera were analyzed by flow cytometry to determine whether haploid DNA content served as a valid criterion for species identification. The DNA content of species often confused with each other due to their overlapping size and geographical occurrence, such as Alexandrium ostenfeldii and the complexes Alexandrium catenella, Alexandrium tamarense, Alexandrium minutum and Alexandrium tamutum, and Karlodinium veneficum and Karlodinium armiger were analyzed. These species differed greatly in DNA content, which provided a means of distinguishing among them. The only cases of DNA overlap involved A. ostenfeldii with Alexandrium peruvianum, and A. catenella with A. tamarense, two groups not yet clearly established either morphologically or genetically. Variability in intraspecies DNA content was observed only in the species K. veneficum. Significant differences between the two A. tamarense strains analyzed were not detected, and the haploid DNA content (63 pg cell−1) was very different from the one reported previously for this species (103.5 pg cell−1), suggesting cryptic speciation within this group. Flow-cytometric analysis of field samples identified K. veneficum as the causative species of a bloom, suggesting this method as a tool to readily identify species responsible for natural blooms. Additionally, after clonal cultures had been established, cytometric analyses corroborated the variability in the haploid DNA content of this species
DescriptionSpecial issue Phytoplankton Life-Cycles and Their Impacts on the Ecology of Harmful Algal Bloom.-- 7 pages, 5 figures, 2 tables
Publisher version (URL)http://dx.doi.org/10.1016/j.dsr2.2009.09.008
URIhttp://hdl.handle.net/10261/21844
DOI10.1016/j.dsr2.2009.09.008
ISSN0967-0645
Appears in Collections:(ICM) Artículos
Files in This Item:
There are no files associated with this item.
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.