English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/21820
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

DC FieldValueLanguage
dc.contributor.authorOrdóñez-Morán, Paloma-
dc.contributor.authorLarriba, María Jesús-
dc.contributor.authorPálmer, Héctor G.-
dc.contributor.authorValero, Ruth A.-
dc.contributor.authorBarbáchano, Antonio-
dc.contributor.authorDuñach, Mireia-
dc.contributor.authorVillalobos, Carlos-
dc.contributor.authorBerciano, María T.-
dc.contributor.authorLafarga, Miguel-
dc.contributor.authorMuñoz Terol, Alberto-
dc.date.accessioned2010-03-02T11:53:34Z-
dc.date.available2010-03-02T11:53:34Z-
dc.date.issued2008-11-17-
dc.identifier.citationJournal of Cell Biology 183(4): 697-719 (2008)en_US
dc.identifier.issn0021-9525-
dc.identifier.urihttp://hdl.handle.net/10261/21820-
dc.description.abstractThe active vitamin D metabolite 1,25-dihydroxy-vitamin D-3 (1,25(OH)(2)D-3) inhibits proliferation and promotes differentiation of colon cancer cells through the activation of vitamin D receptor (VDR), a transcription factor of the nuclear receptor superfamily. Additionally, 1,25(OH)(2)D-3 has several nongenomic effects of uncertain relevance. We show that 1,25(OH)(2)D-3 induces a transcription-independent Ca2+ influx and activation of RhoA-Rho-associated coiled kinase (ROCK). This requires VDR and is followed by activation of the p38 mitogen-activated protein kinase (p38MAPK) and mitogen- and stress-activated kinase 1 (MSK1). As shown by the use of chemical inhibitors, dominant-negative mutants and small interfering RNA, RhoA-ROCK, and p38MAPK-MSK1 activation is necessary for the induction of CDH1/E-cadherin, CYP24, and other genes and of an adhesive phenotype by 1,25(OH)(2)D-3. RhoA-ROCK and MSK1 are also required for the inhibition of Wnt-beta-catenin pathway and cell proliferation. Thus, the action of 1,25( OH) 2 D 3 on colon carcinoma cells depends on the dual action of VDR as a transcription factor and a nongenomic activator of RhoA-ROCK and p38MAPK-MSK1en_US
dc.description.sponsorshipMinisterio de Ciencia e Innovacion SAF2007-60341 Ministerio de Sanidad y Consumo (Instituto de Salud Carlos III-Redes Tematicas de Investigacion Cooperativa) RD06/0020/0009 Comunidad de Madrid S-GEN-0266/2006 European Union MRTN-CT-2005-019496en_US
dc.format.extent4075108 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherRockefeller University Pressen_US
dc.rightsopenAccessen_US
dc.subjectD-receptoren_US
dc.subjectSignaling pathwaysen_US
dc.subjectColorectal-canceren_US
dc.subject1-alphaen_US
dc.subject25-dihydroxyvitamin d-3en_US
dc.subjectMessenger-rnaen_US
dc.subjectIn-vitroen_US
dc.titleRhoA-ROCK and p38MAPK-MSK1 mediate vitamin D effects on gene expression, phenotype, and Wnt pathway in colon cancer cellsen_US
dc.typeartículoen_US
dc.identifier.doi10.1083/jcb.200803020-
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://dx.doi.org/10.1083/jcb.200803020en_US
Appears in Collections:(IIBM) Artículos
(IBGM) Artículos
Files in This Item:
File Description SizeFormat 
RhoA-ROCK.pdf3,98 MBAdobe PDFThumbnail
View/Open
Show simple item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.