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Título: | Potentiometric detection of ATP based on the transmembrane proton gradient generated by ATPase reconstituted on a gold electrode |
Autor: | García-Molina, Gabriel; Natale, Paolo; Valenzuela, Laura; Álvarez-Malmagro, Julia; Gutiérrez Sánchez, Cristina; Iglesias Juez, Ana CSIC ORCID; López-Montero, Iván; Vélez, Marisela CSIC ORCID; Pita, Marcos CSIC ORCID ; López de Lacey, Antonio CSIC ORCID | Palabras clave: | ATP ATP-synthase Potentiometric biosensor Biomimetic membranes |
Fecha de publicación: | 2020 | Editor: | Elsevier | Citación: | Bioelectrochemistry 133 (2020) | Resumen: | [EN] Adenosine triphosphate (ATP) is a key molecule as energy vector for living organisms, therefore its detection reveals the presence of microbial colonies. Environments where the existence of microbial pathogens suppose a health hazard can benefit from real time monitoring of such molecule. We report a potentiometric biosensor based on ATP-synthase from Escherichia coli reconstituted in a floating phospholipid bilayer over gold electrodes modified with a 4-aminothiophenol self-assembled monolayer. The use of a pH-dependent redox probe on the electrode surface allows a simple, specific and reliable on site determination of ATP concentration from 1 μM to 1 mM. The broad range ATP biosensor can offer an alternative way of measuring in a few minutes the presence of microbial contamination. | Versión del editor: | http://dx.doi.org/10.1016/j.bioelechem.2020.107490 | URI: | http://hdl.handle.net/10261/216241 | DOI: | 10.1016/j.bioelechem.2020.107490 | Identificadores: | doi: 10.1016/j.bioelechem.2020.107490 e-issn: 1878-562X issn: 1567-5394 |
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