Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/21618
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Tingaud-Sequeira, Angèle | - |
dc.contributor.author | Zapater, Cinta | - |
dc.contributor.author | Chauvigné, François | - |
dc.contributor.author | Otero, David | - |
dc.contributor.author | Cerdà, Joan | - |
dc.date.accessioned | 2010-02-25T11:31:54Z | - |
dc.date.available | 2010-02-25T11:31:54Z | - |
dc.date.issued | 2009-04 | - |
dc.identifier.citation | American Journal Physiology Regulation Integrative and Comparative Physiology 296(4): R1041–R1052 (2009) | en_US |
dc.identifier.issn | 0363-6119 | - |
dc.identifier.uri | http://hdl.handle.net/10261/21618 | - |
dc.description | 13 pages, 7 figures, 3 tables | en_US |
dc.description.abstract | Embryos of the marine killifish Fundulus heteroclitus are adapted to survive aerially. However, it is unknown if they are able to control development under dehydration conditions. Here, we show that air-exposed blastula embryos under saturated relative humidity were able to stimulate development, and hence the time of hatching was advanced with respect to embryos continuously immersed in seawater. Embryos exposed to air at later developmental stages did not hatch until water was added, while development was not arrested. Air-exposed embryos avoided dehydration probably because of their thickened egg envelope, although it suffered significant evaporative water loss. The potential role of aquaporins as part of the embryo response to dehydration was investigated by cloning the aquaporin-0 (FhAqp0), -1a (FhAqp1a), and -3 (FhAqp3) cDNAs. Functional expression in Xenopus laevis oocytes showed that FhaAqp1a was a water-selective channel, whereas FhAqp3 was permeable to water, glycerol, and urea. Expression of fhaqp0 and fhaqp1a was prominent during organogenesis, and their mRNA levels were similar between water- and air-incubated embryos. However, fhaqp3 transcripts were highly and transiently accumulated during gastrulation, and the protein product was localized in the basolateral membrane of the enveloping epithelial cell layer and in the membrane of ingressing and migrating blastomers. Interestingly, both fhaqp3 transcripts and FhAqp3 polypeptides were downregulated in air-exposed embryos. These data demonstrate that killifish embryos respond adaptively to environmental desiccation by accelerating development and that embryos are able to transduce dehydration conditions into molecular responses. The reduced synthesis of FhAqp3 may be one of these mechanisms to regulate water and/or solute transport in the embryo. | en_US |
dc.description.sponsorship | This study was supported by the European Commission New and Emerging Science and Technologies (NEST) program (contract no. 012674-2 Sleeping Beauty) and by a grant from the Spanish Ministry of Education and Science (MEC; AGL2004-00316/ACU) to J. Cerda`. Participation of C. Zapater and F. Chauvigne´ was financed by a predoctoral fellowship from MEC (Spain) and by the European Commission [Marie Curie Research Training Network Aqua (glycero)porins, MRTN-CT-2006-035995], respectively. | en_US |
dc.format.extent | 22195 bytes | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | en_US |
dc.publisher | American Physiological Society | en_US |
dc.rights | closedAccess | en_US |
dc.subject | Fish | en_US |
dc.subject | Desiccation | en_US |
dc.subject | Hatching | en_US |
dc.subject | Egg envelope | en_US |
dc.subject | Gastrulation | en_US |
dc.subject | Aquaporin-0 | en_US |
dc.subject | Aquaporin-1 | en_US |
dc.subject | Aquaporin-3 | en_US |
dc.title | Adaptive plasticity of killifish (Fundulus heteroclitus) embryos: dehydration-stimulated development and differential aquaporin-3 expression | en_US |
dc.type | artículo | en_US |
dc.identifier.doi | 10.1152/ajpregu.91002.2008 | - |
dc.description.peerreviewed | Peer reviewed | en_US |
dc.relation.publisherversion | https://doi.org/10.1152/ajpregu.91002.2008 | en_US |
dc.identifier.e-issn | 1522-1490 | - |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | none | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | No Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (ICM) Artículos |
CORE Recommender
SCOPUSTM
Citations
50
checked on 13-abr-2024
WEB OF SCIENCETM
Citations
50
checked on 29-feb-2024
Page view(s)
390
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.