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Title

Electrical Modeling of the Growth and Di erentiation of Skeletal Myoblasts Cell Cultures for Tissue Engineering

AuthorsOlmo, Alberto; Yuste, Yaiza; Serrano, Juan A.; Maldonado-Jacobi, Andrés; Pérez, Pablo ; Huertas, Gloria ; Pereira, Sheila; Yufera, Alberto; Portilla, Fernando de la
KeywordsOscillation-based test
Skeletal muscle
Stem cell differentiation
Impedance spectroscopy
Electrical modeling
Issue Date2-Jun-2020
PublisherMultidisciplinary Digital Publishing Institute
CitationSensors, 20,:3152 (2020)
AbstractIn tissue engineering, of utmost importance is the control of tissue formation, in order to form tissue constructs of clinical relevance. In this work, we present the use of an impedance spectroscopy technique for the real-time measurement of the dielectric properties of skeletal myoblast cell cultures. The processes involved in the growth and di erentiation of these cell cultures in skeletal muscle are studied. A circuit based on the oscillation-based test technique was used, avoiding the use of high-performance circuitry or external input signals. The e ect of electrical pulse stimulation applied to cell cultures was also studied. The technique proved useful for monitoring in real-time the processes of cell growth and estimating the fill factor of muscular stem cells. Impedance spectroscopy was also useful to study the real-time monitoring of cell di erentiation, obtaining di erent oscillation amplitude levels for di erentiated and undi erentiated cell cultures. Finally, an electrical model was implemented to better understand the physical properties of the cell culture and control the tissue formation process
Publisher version (URL)https://doi.org/10.3390/s20113152
URIhttp://hdl.handle.net/10261/214325
DOIhttp://dx.doi.org/10.3390/s20113152
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