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In vitro study of the rumen and hingut fermentation of starch and cellulose

AuthorsRanilla, María José CSIC ORCID ; Carro Travieso, Mª Dolores CSIC ORCID; Giráldez, Francisco Javier CSIC ORCID ; Mantecón, Ángel R. CSIC ORCID
Issue Date2004
PublisherEDP Sciences
Institut national de la recherche agronomique (France)
CitationReproduction, Nutrition, Development, 44 (Suppl. 1), S74 (2004)
AbstractThe ruminal and caecal contents from seven lambs fed a concentrate diet were used to study in vitro fermentation. Four hour incubations were performed without added substrate or with starch and cellulose. There were differences between ruminal and caecal fermentation for most of the studied parameters. Total VFA production and the Ac/Pr ratio were higher (P < 0.001) with caecal than with ruminal inocula in the absence of substrate (721 vs. 261 μmol and 3.65 vs. 2.64, for caecum and rumen, respectively). When starch was incubated with ruminal fluid, total VFA production almost doubled (P < 0.001) the one obtained with caecal contents (3048 vs. 1655 μmol, respectively). Fermentation pattern was also different for both inocula, with lower (P < 0.001) acetate and butyrate and higher (P < 0.001) propionate molar proportions with ruminal fluid when starch was incubated. Methane production was higher (P < 0.05) with ruminal than with caecal fluid (259 vs. 164 μmol, respectively) resulting in a higher (P < 0.01) hydrogen recovery. For cellulose, total VFA production and Ac/Pr ratio were higher (P < 0.01) in the caecal fermentation (697 vs. 320 μmol and 3.73 vs. 1.99, caecum vs. rumen). Molar proportions of the main VFA followed the same trend as with starch. Methane production was higher (P < 0.01) when cellulose was incubated with caecal contents (35.6 vs. 14.2 μmol, caecum vs. rumen), but hydrogen recovery was lower (P < 0.01) than that calculated for ruminal fluid. The results point to a high difference between rumen and hindgut fermentation when both inocula were used for in vitro incubations, showing that the first cannot serve as a model for the stoichiometry of the second.
DescriptionSpecial Issue: Fouth Joint INRA-RRI.-- Contributed to: Symposium Gut Microbiology (Clermont-Feerand, France, Jun 21-24, 2004).
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Appears in Collections:(IGM) Comunicaciones congresos

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