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Title

Atorvastatin-induced endothelial nitric oxide synthase expression in endothelial cells is mediated by endoglin

AuthorsZemankova, L.; Varejckova, Michala; Dolezelova, Eva; Fikrova, Petra; Jezkova, Katerina; Rathouska, Jana; Červený, Lukáš; Botella, Luisa María ; Bernabéu, Carmelo ; Nachtigal, Petr
KeywordsEndothelial cells
Atorvastatin
Endothelial nitric oxide synthase
Endoglin
Human umbilical vein endothelial cells
Small interfering RNA
Tumor necrosis factor-alpha
Transforming growth factor-beta
Reactive oxygen species
Issue DateJun-2015
PublisherPolish Physiological Society
CitationJ Physiol Pharmacol 66(3) 403-13 (2015)
AbstractEndoglin, a transforming growth factor β (TGF-β) receptor type III, is co-expressed with endothelial nitric oxide synthase (eNOS) in aortic endothelium in atherosclerotic plaques of mice. Interestingly, atorvastatin (ATV) is able to increase both endoglin and eNOS expression and reduce plaque size beyond its lipid lowering effects but by unknown mechanisms. We hypothesized whether inflammation modulates ATV-dependent induction of endoglin and eNOS expression in vitro in endothelial cells and whether ATV-induced eNOS expression is regulated via endoglin. After treatment of human umbilical vein endothelial cells (HUVECs) with TNF-α, endoglin and eNOS protein expression was reduced, concomitantly with increased levels of cell surface VCAM-1 and soluble endoglin, as determined by flow cytometry, Western blot and ELISA analyses. By contrast, ATV treatment increased endoglin and eNOS protein expression, while preventing TNF-α-mediated downregulation of endoglin and eNOS protein levels. Moreover, suppression of endoglin using small interfering RNA (siRNA), but not inhibition of TGF-β signaling with SB431542, abrogated ATV-induced eNOS expression. These results suggest that ATV treatment prevents inflammation-reduced endoglin and eNOS expression in endothelial cells and that ATV-induced eNOS expression strongly depends on the proper expression of endoglin in HUVECs. Possible implications of these findings might be reflected in pathological conditions characterized by reduced expression of endoglin and eNOS as for example in hereditary hemorrhagic telangiectasia or in other endothelial dysfunctions.
Description11 p.-6 fig.
Publisher version (URL)http://www.jpp.krakow.pl/journal/archive/06_15/articles/09_article.html
URIhttp://hdl.handle.net/10261/213043
ISSN08675910
18991505
Appears in Collections:(CIB) Libros y partes de libros
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