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Title

Antihypertensive effects of virgin olive oil (Unfiltered) low molecular weight peptides with ace inhibitory activity in spontaneously hypertensive rats

AuthorsAlcaide Hidalgo, Juan Maria; Romero, M.; Duarte, J.; López-Huertas, León Eduardo
KeywordsUnfiltered virgin olive oil
Peptides
ACE
Hypertension
Spontaneously hypertensive rats
Issue Date2020
CitationNutrients 12(1), 271 (2020)
AbstractThe low molecular weight peptide composition of virgin olive oil (VOO) is mostly unknown. We hypothesised that unfiltered VOO could possess low molecular weight peptides with antihypertensive activity. We produced unfiltered VOO and obtained a water-soluble peptide extract from it. The peptides were separated by size-exclusion using fast protein liquid chromatography, and the low molecular weight fraction was analysed by nanoscale liquid chromatography-Orbitrap coupled with tandem mass spectrometry and de novo sequencing. We selected 23 peptide sequences containing between 6 and 9 amino acids and molecular masses ranging 698–1017 Da. Those peptides were chemically synthesised and their angiotensin-converting enzyme (ACE) inhibitory activity was studied in vitro. Seven peptides showed a strong activity, with half maximal inhibitory concentration (IC50) <10 μm. The antihypertensive effects of the four most active synthesised ACE inhibitor peptides were studied in spontaneously hypertensive rats (SHR). Acute oral administration of synthetic peptides RDGGYCC and CCGNAVPQ showed antihypertensive activity in SHR. We conclude that unfiltered VOO naturally contains low molecular weight peptides with specific ACE inhibitory activity and antihypertensive effects in SHR.
Publisher version (URL)http://dx.doi.org/10.3390/nu12010271
URIhttp://hdl.handle.net/10261/212360
Identifiersdoi: 10.3390/nu12010271
issn: 2072-6643
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