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dc.contributor.authorTaladriz, Sorayaes_ES
dc.contributor.authorGonzález-Aseguinolazaes_ES
dc.contributor.authorMarquet, Albertoes_ES
dc.contributor.authorLarraga, Vicentees_ES
dc.date.accessioned2020-05-25T11:48:16Z-
dc.date.available2020-05-25T11:48:16Z-
dc.date.issued1999-01-29-
dc.identifier.citationFEBS Lett 443 (3) 375-80 (1999)es_ES
dc.identifier.issn0014-5793-
dc.identifier.urihttp://hdl.handle.net/10261/212119-
dc.description6 p.-6 fig.es_ES
dc.description.abstractThe family of the RACK molecules (receptors for activated C kinases) are present in all the species studied so far. In the genus Leishmania, these molecules also induce a strong immune reaction against the infection. We have cloned and characterised the gene that encodes the RACK analogue from the parasite trypanosomatid Crithidia fasciculata (CACK). The molecule seems to be encoded by two genes. The sequence analysis of the cloned open reading frame indicates the existence of a high degree of conservation not only with other members of the Trypanosomatidae but also with mammalians. The study of the protein kinase C phosphorylation sites shows the presence of three of them, shared with the mammalian species, additional to those present in the other protozoa suggesting a certain phylogenetic distance between the protozoon Crithidia fasciculata and the rest of the Trypanosomatidae. The CACK-encoded polypeptide shows an additional sequence of four amino acids at the carboxy-terminal end, which produces a different folding of the fragment with the presence of an alpha-helix instead of the beta-sheet usual in all the other species studied. A similar result is elicited at the amino-terminal end by the change of three amino acid residues. The immunolocalisation experiments show that the CACK displays a pattern with a distribution mainly at the plasma membrane, different from that of the related Leishmania species used as control, that displays a distribution close to the nucleus. Altogether, the data suggest that the existence of the structural differences found may have functional consequences.es_ES
dc.description.sponsorshipThis work has been supported by Grant 97/0492 from the Fondo de Investigación Sanitaria (FIS) of the Ministry of Health.es_ES
dc.language.isoenges_ES
dc.publisherJohn Wiley & Sonses_ES
dc.rightsclosedAccesses_ES
dc.subjectp36 RACK analoguees_ES
dc.subjectProtein kinase C activationes_ES
dc.subjectCrithidia fasciculataes_ES
dc.titleCloning, molecular analysis and differential cell localisation of the p36 RACK analogue antigen from the parasite protozoon Crithidia fasciculataes_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/s0014-5793(99)00006-x-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/s0014-5793(99)00006-xes_ES
dc.identifier.e-issn1873-3468-
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100004587es_ES
dc.contributor.orcidGonzález-Aseguinolaza, Gloria [0000-0002-1600-4562]es_ES
dc.contributor.orcidLarraga, Vicente [0000-0003-1260-7400]es_ES
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