English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/211988
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

DNA polymerase beta mRNA determination by relative quantitative RT-PCR from Leishmania infantum intracellular amastigotes

AuthorsRamiro Ibáñez, M. J.; Hanke, Tobias; Taladriz, Soraya; Larraga, Vicente
KeywordsInfected macrophage
Intracellular form
Intracellular amastigote
Degradation problem
Classic gradient
Issue DateAug-2002
PublisherSpringer
CitationParasitol Res 88 (8) 760–767 (2002)
AbstractGene expression level is extremely difficult to assess in the intracellular form of Leishmania infantum, the amastigote, due to host mRNA contamination, low supply of parasites and stress degradation problems. We obtained and analyzed L. infantum DNA polymerase β (Li Pol β) , a suitable enzyme for differential expression studies. The amount of Li Pol β mRNA was determined in different forms of the parasite (extracellular promastigote, intracellular amastigote) by Northern blot and by relative quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Amastigote transcript levels were determined from both gradient-purified parasites and directly from a population of infected macrophages. The mRNA, undetectable when obtained from amastigotes by the classic gradient centrifugation method and subsequent Northern analysis, was clearly and specifically detectable by this quantitative RT-PCR method from the mixed macrophage/parasite cell population. Li Pol β displays a different pattern of expression in the distinct forms of the parasite cycle that correlate with the infectivity of the protozoon. Thus, Li Pol β mRNA expression is developmentally regulated, being clearly higher in the more infective forms of the parasite.
Description8 p.-5 fig.
Publisher version (URL)https://doi.org/10.1007/s00436-002-0653-0
URIhttp://hdl.handle.net/10261/211988
DOI10.1007/s00436-002-0653-0
ISSN0932-0113
E-ISSN1432-1955
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdfRestringido15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.