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Título: | Understanding the paradoxical mechanical response of in-phase A-tracts at different force regimes |
Autor: | Marin-González, Alberto; Pastrana, César L.; Bocanegra, Rebeca; Martín González, Alejandro; Vilhena, J. G. CSIC ORCID; Pérez, Rubén; Ibarra, Borja; Aicart-Ramos, Clara; Moreno-Herrero, Fernando | Fecha de publicación: | may-2020 | Editor: | Oxford University Press | Citación: | Nucleic Acids Research 48(9): 5024-5036 (2020) | Resumen: | A-tracts are A:T rich DNA sequences that exhibit unique structural and mechanical properties associated with several functions i n v i vo . The crystallographic structure of A-tracts has been well characterized. However, the mechanical properties of these sequences is controversial and their response to force remains unexplored. Here, we rationalize the mechanical properties of in-phase A-tracts present in the C a e n o r h a b d i t i s e l e g a n s genome over a wide range of external forces, using single-molecule experiments and theoretical polymer models. Atomic Force Microscopy imaging shows that A-tracts induce long-range (∼200 nm) bending, which originates from an intrinsically bent structure rather than from larger bending flexibility. These data are well described with a theoretical model based on the worm-like chain model that includes intrinsic bending. Magnetic tweezers experiments show that the mechanical response of A-tracts and arbitrary DNA sequences have a similar dependence with monovalent salt supporting that the observed A-tract bend is intrinsic to the sequence. Optical tweezers experiments reveal a high stretch modulus of the A-tract sequences in the enthalpic regime. Our work rationalizes the complex multiscale flexibility of A-tracts, providing a physical basis for the versatile character of these sequences inside the cell. | Versión del editor: | https://doi.org/10.1093/nar/gkaa225 | URI: | http://hdl.handle.net/10261/211579 | DOI: | 10.1093/nar/gkaa225. | ISSN: | 0305-1048 | E-ISSN: | 1362-4962 |
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MORENO-HERRERO, Fernando - Nucleic Acids Research - 2020.pdf | Artículo pincipal | 1,84 MB | Adobe PDF | Visualizar/Abrir |
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