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dc.contributor.authorCouso, Inmaculada-
dc.contributor.authorPérez-Pérez, María Esther-
dc.contributor.authorFord, Megan M.-
dc.contributor.authorMartínez-Force, Enrique-
dc.contributor.authorHicks, Leslie M.-
dc.contributor.authorUmen, James G.-
dc.contributor.authorCrespo, José L.-
dc.identifierdoi: 10.1105/tpc.19.00179-
dc.identifiere-issn: 1532-298X-
dc.identifierissn: 1040-4651-
dc.identifier.citationPlant Cell 32: 69- 78 (2020)-
dc.description5 Figuras-
dc.description.abstractTarget of rapamycin complex 1 (TORC1) is a central regulator of cell growth. It balances anabolic and catabolic processes in response to nutrients, growth factors, and energy availability. Nitrogen- and carbon-containing metabolites have been shown to activate TORC1 in yeast, animals, and plants. Here, we show that phosphorus (P) regulates TORC1 signaling in the model green alga Chlamydomonas (Chlamydomonas reinhardtii) via LST8, a conserved TORC1 subunit that interacts with the kinase domain of TOR. P starvation results in a sharp decrease in LST8 abundance and downregulation of TORC1 activity. A hypomorphic lst8 mutation resulted in decreased LST8 abundance, and it both reduced TORC1 signaling and altered the cellular response to P starvation. Additionally, we found that LST8 levels and TORC1 activity were not properly regulated in a mutant defective in the transcription factor PSR1, which is the major mediator of P deprivation responses in Chlamydomonas. Unlike wild-type cells, the psr1 mutant failed to downregulate LST8 abundance and TORC1 activity when under P limitation. These results identify PSR1 as an upstream regulator of TORC1 and demonstrate that TORC1 is a key component in P signaling in Chlamydomonas.-
dc.description.sponsorshipThis work was supported in part by the Ministerio de Economía y Competitividad (grants BFU2015-68216-P and PGC2018-099048-B-100 to J.L.C. and grant BIO2015-74432-JIN to M.E.P.-P.) and the National Science Foundation (CAREER award MCB-1552522 to L.M.H. and grant MCB-1616820 to J.G.U.). I.C. is the recipient of an MSCA-IF-EF-RI contract (grant number 750996) from the European Commission.-
dc.publisherAmerican Society of Plant Physiologists-
dc.titlePhosphorus Availability Regulates TORC1 Signaling via LST8 in Chlamydomonas.-
dc.contributor.funderMinisterio de Economía y Competitividad (España)-
dc.contributor.funderNational Science Foundation (US)-
dc.contributor.funderEuropean Commission-
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