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Título: | The enzymatic paper-on-a-chip concept: Towards the on-site organophosphate and carbamate pesticides detection in fruits and vegetables |
Autor: | Tsagkaris, Aristeidis; Filippini, Danie; Salvador, Juan Pablo CSIC ORCID; Marco, María Pilar CSIC ORCID CVN ; Pulkrábová, Jana; Hajslova, Jana | Fecha de publicación: | 21-jul-2019 | Resumen: | Lab-on-a-chip (LOC) devices is a concept of increasing popularity because it provides miniaturization, integrated sample handling and low-cost measurement. Although the determination of organophosphate and carbamate pesticides requires expensive and time-consuming chromatographic methods, rapid screening can be achieved based on the principle that organophosphates and carbamates inhibit acetylcholinesterase (AChE). In-vitro, AChE can hydrolyze certain colorless substrates to color products. However in the presence of an inhibitor, AChE is inhibited dependently on a pesticide concentration and this inhibition can be correlated to a color intensity decrease. To this end, we developed an interdisciplinary analytical platform for organophosphates and carbamates detection. To begin with, AChE was physically adsorbed on Whatman paper. Then, two AChE strips were incorporated in a prototype LOC creating a so called enzymatic paper-on-a-chip (EPOC) device. The EPOC device provided integrated samples and substrates loading using silicon tubes as a pumping element eliminating the pipetting need. To monitor the color development on AChE strips, the EPOC device was placed in an in-house 3D-printed smartphone reader. Paper assay images were captured using the OpenCamera application and image data processing was performed in MatLab. Finally, the developed platform was successfully used to quantitatively detect carbofuran in apple extracts at sub-ppm level. Despite being a restricted pesticide in the EU, carbofuran was recorded among the pesticides with frequent MRL exceedances in the latest EFSA report indicating the need for rapid screening methods. All in all, this fast, simple and cost-effective approach can be a paradigm shift towards the on-site chemical residues detection. | URI: | http://hdl.handle.net/10261/210081 |
Aparece en las colecciones: | (IQAC) Comunicaciones congresos |
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