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Mobility of iron oxide nanoparticles in an extracellular matrix and their internalization in patient-derived colorectal cancer organoids for magnetic hyperthermia treatments

AuthorsMorales, I.; Fernández-Barral, A. ; Costales-Carrera, Alba; Barbáchano, Antonio ; Márquez M. A.; Morales, M. P. ; Hernando, Antonio ; Muñoz Terol, Alberto ; Presa, Patricia de la
Issue Date6-Jun-2019
CitationSpanish Conference on Biomedical Applications of Nanomaterials (SBAN) (2019)
AbstractIn this study, we investigate the uptake of magnetic nanoparticles in colon cancer cells grown in 3D in order to optimize the magnetic hyperthermia performance. Most of the studies related to cellular uptake have been reported in 2D cells grown in monolayers on a Petri dish, and it is necessary to study a more realistic cell culture system that may resemble the in vivo conditions. For that purpose, human colon cancer cells were embedded in a matrix (Matrigel) that allows them grow in 3D. These structures are called organoids, and they are able to acquire the architecture and function of origin tissue. First of all, we studied the interaction of iron oxide nanoparticles with the extracellular medium. The permeability of Matrigel was investigated by putting nanoparticles on Matrigel’s surface and analysing their diffusion and the temporal evolution of the depth of the colloid by means of a phase-contrast microscope. Once the Matrigel barrier had been crossed, the internalization of nanoparticles with different coatings was investigated under a confocal microscope. The internalization is discussed as a function of sizes and aggregation degree.
DescriptionTrabajo presentado en "Spanish Conference on Biomedical Applications of Nanomaterials", celebrada en Madrid (España) del 6 al 7 de junio de 2019.
Appears in Collections:(ICMM) Comunicaciones congresos
(IIBM) Comunicaciones congresos
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