Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/20884
COMPARTIR / EXPORTAR:
SHARE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Centromere-localized breaks indicate the generation of DNA damage by the mitotic spindle |
Autor: | Alonso Guerrero, Astrid; Cano Gamero, Mercedes; Trachana, Varvara; Fütterer, Agnes; Pacios-Bras, Cristina; Panadero Díaz-Concha, Nuria; Cigudosa, Juan C.; Martínez-Alonso, Carlos; Van Wely, Karel H. M. | Palabras clave: | Centrosomes Chromosome instability Bouble-Strand Break H2A.X |
Fecha de publicación: | 8-feb-2010 | Editor: | National Academy of Sciences (U.S.) | Citación: | PNAS. Proceedings of the National Academy of Sciences of the United States of America 107(9): 4159-4164 (2010) | Serie: | PNAS 0912143106 |
Resumen: | Most carcinomas present some form of chromosome instability in combination with spindle defects. Numerical instability is likely caused by spindle aberrations, but the origin of breaks and translocations remains elusive. To determine whether one mechanism can bring about both types of instability, we studied the relationship between DNA damage and spindle defects. Although lacking apparent repair defects, primary Dido mutant cells formed micronuclei containing damaged DNA. The presence of centromeres showed that micronuclei were caused by spindle defects, and cell cycle markers showed that DNA damage was generated during mitosis. Although the micronuclei themselves persisted, the DNA damage within was repaired during S and G2 phases. DNA breaks in Dido mutant cells regularly colocalized with centromeres, which were occasionally distorted. Comparable defects were found in APC mutant cell lines, an independent system for spindle defects. On the basis of these results, we propose a model for break formation in which spindle defects lead to centromere shearing. | Versión del editor: | doi:10.1073/pnas.0912143106 | URI: | http://hdl.handle.net/10261/20884 | ISSN: | 1091-6490 |
Aparece en las colecciones: | (CNB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
PNAS_2010_AAG.pdf | 1,56 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
Page view(s)
497
checked on 18-abr-2024
Download(s)
310
checked on 18-abr-2024
Google ScholarTM
Check
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.