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Título: | Melanopsin for precise optogenetic activation of astrocyte-neuron networks |
Autor: | Mederos, Sara CSIC ORCID; Hernández-Vivanco, Alicia CSIC ORCID; Ramírez-Franco, Jorge CSIC ORCID; Martín-Fernández, Mario; Navarrete, Marta CSIC ORCID ; Yang, A.; Boyden, E.S.; Perea, Gertrudis CSIC ORCID | Palabras clave: | Astrocytes optogenetics melanopsin neuron–glia interactions synaptic plasticity |
Fecha de publicación: | 2019 | Editor: | John Wiley & Sons | Citación: | Glia 67: 915- 934 (2019) | Resumen: | Optogenetics has been widely expanded to enhance or suppress neuronal activity and it has been recently applied to glial cells. Here, we have used a new approach based on selective expression of melanopsin, a G-protein-coupled photopigment, in astrocytes to trigger Ca2+ signaling. Using the genetically encoded Ca2+ indicator GCaMP6f and two-photon imaging, we show that melanopsin is both competent to stimulate robust IP3-dependent Ca2+ signals in astrocyte fine processes, and to evoke an ATP/Adenosine-dependent transient boost of hippocampal excitatory synaptic transmission. Additionally, under low-frequency light stimulation conditions, melanopsin-transfected astrocytes can trigger long-term synaptic changes. In vivo, melanopsin-astrocyte activation enhances episodic-like memory, suggesting melanopsin as an optical tool that could recapitulate the wide range of regulatory actions of astrocytes on neuronal networks in behaving animals. These results describe a novel approach using melanopsin as a precise trigger for astrocytes that mimics their endogenous G-protein signaling pathways, and present melanopsin as a valuable optical tool for neuron-glia studies. | Versión del editor: | http://dx.doi.org/10.1002/glia.23580 | URI: | http://hdl.handle.net/10261/207658 | DOI: | 10.1002/glia.23580 | Identificadores: | doi: 10.1002/glia.23580 issn: 0894-1491 |
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