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Título: | Characterization of genes associated with induced resistance against Penicillium expansum in apple fruit treated with quercetin |
Autor: | Sanzani, Simona Marianna; Schena, Leonardo; Girolamo, Annalisa de; Ippolito, Antonio; González-Candelas, Luis CSIC ORCID CVN | Palabras clave: | Induced disease resistance Quercetin Penicillium expansum Malus domestica Quantitative real-time PCR Suppression subtractive hybridization |
Fecha de publicación: | abr-2010 | Editor: | Elsevier | Citación: | Postharvest Biology and Technology 56 (1): 1-11 (2010) | Resumen: | Penicillium expansum causes blue mould, a serious postharvest disease of apples, and is the main producer of the mycotoxin patulin. Since control by synthetic fungicides is less accepted by consumers, the demand for alternative means is pressing. In a recent study, the flavonoid quercetin, although scarcely effective in in vitro assays against P. expansum growth, significantly reduced blue mould rots on ‘Golden Delicious’ apples, suggesting an enhancement of host disease resistance. To confirm or reject this hypothesis, genes differentially expressed in quercetin-treated ‘Golden Delicious’ apples were identified by suppression subtractive hybridization (SSH). A pool of 88 unique gene transcripts was obtained. Several sequences revealed high similarities with different classes of pathogenesis-related proteins (RNase-like PR10 and PR8), or with proteins expressed under stress conditions. Other transcripts had high similarity to genes of unknown function or genes coding for proteins having a role in pathogen recognition and in signalling pathways. SSH data were validated by analysing the expression of 14 genes by quantitative real-time PCR (qPCR). Eleven genes proved to be up-regulated at a medium-high level in freshly harvested apples. Among these, 5 genes selected for temporal expression profiling revealed the existence of a combined effect, particularly at 24 or 48 h, between wounding and phenolic treatment. These results provide evidence that quercetin induces resistance to P. expansum in apples, by acting on the transcription level of genes involved in several distinct metabolic processes. | Descripción: | 11 pages, 4 tables, 5 figures.-- Online version available 29 December 2009 | Versión del editor: | http://dx.doi.org/10.1016/j.postharvbio.2009.11.010 | URI: | 10261/20725 | DOI: | 10.1016/j.postharvbio.2009.11.010 | ISSN: | 0925-5214 |
Aparece en las colecciones: | (IATA) Artículos |
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Sanzani_et_al_PBT 2010 revised version.pdf | 634,06 kB | Adobe PDF | Visualizar/Abrir |
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