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Crystallization and preliminary crystallographic characterization of the extrinsic PsbP protein of photosystem II from Spinacia oleracea

AuthorsKohoutova, Jaroslava; Kuta-Smatanova, Ivana; Brynda, Jiri; Lapkouski, Mikalai; Revuelta Doval, José Luis ; Arellano, Juan B. ; Ettrich, Rüdiger
KeywordsPhotosystem II
Oxygen Evolving Complex
Crystal structure
Spinacia oleracea
Issue DateFeb-2009
PublisherBlackwell Publishing
CitationActa Cryst. (2009). F65:111–115
AbstractPreliminary X-ray diffraction analysis of the extrinsic PsbP protein of photosystem II from spinach (Spinacia oleracea) was performed using N-terminally His-tagged recombinant PsbP protein overexpressed in Escherichia coli. Recombinant PsbP protein (thrombin-digested recombinant His-tagged PsbP) stored in bis-Tris buffer pH 6.00 was crystallized using the sitting-drop vapour diffusion technique with PEG 550 MME as a precipitant and zinc sulfate as an additive. SDS–PAGE analysis of a dissolved crystal showed that the crystals did not contain the degradation products of recombinant PsbP protein. PsbP crystals diffracted to 2.06 A resolution in space group P2(1)2(1)2(1), with unit-cell parameters a = 38.68, b = 46.73, c = 88.9 A.
Publisher version (URL)http://journals.iucr.org/f/issues/2009/02/00/issconts.html
Appears in Collections:(IRNASA) Artículos
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