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Título: | Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay |
Autor: | Galindo-Pumariño, Cristina; Herrera, Alberto; Muñoz Terol, Alberto CSIC ORCID; Carrato, Alfredo; Herrera, Mercedes; Peña, Cristina | Fecha de publicación: | 2019 | Editor: | Journal of Visualized Experiments | Citación: | Journal of visualized experiments : JoVE 154: e60304 (2019) | Resumen: | The extracellular matrix (ECM) is a three-dimensional scaffold that acts as the main support for cells in tissues. Besides its structural function, the ECM also participates in cell migration, proliferation, and differentiation. Fibroblasts are the main type of cells modifying ECM fiber arrangement and production. In cancer, CAFs (cancer associated fibroblasts) are in permanent activation status, participating in ECM remodeling, facilitating tumor cell migration, and stimulating tumor-associated angiogenesis, among other pro-tumorigenic roles. The objective of this method is to create a three-dimensional matrix with a fiber composition that is similar to in vivo matrices, using immortalized fibroblasts or human primary CAFs. Fibroblasts are cultured in pre-treated cell culture plates and grown under ascorbic acid stimulation. Then, fibroblasts are removed and matrices are blocked for further cell seeding. In this ECM model, fibroblasts can be activated or modified to generate different kinds of matrix, whose effects can be studied in cell culture. 3D matrices are also shaped by cell signals, like degradation or cross-linking enzymes that might modify fiber distribution. In this context, angiogenesis can be studied, along with other cell types such as epithelial tumor cells. | Versión del editor: | http://dx.doi.org/10.3791/60304 | URI: | http://hdl.handle.net/10261/206683 | DOI: | 10.3791/60304 | ISSN: | 1940-087X |
Aparece en las colecciones: | (IIBM) Artículos |
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