English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/206096
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


MS tools for a systematic approach in survey for carotenoids and their common metabolites

AuthorsPérez Gálvez, Antonio ; Sánchez-García, A.; Garrido Fernández, J. ; Ríos, José Julián
Issue Date2018
CitationArchives of Biochemistry and Biophysics 650: 85- 92 (2018)
AbstractMetabolism of the carotenoids in humans is often reserved to bioconversion of provitamin A carotenoids in retinoids and oxidative/degradative products arising from enzymatic eccentric cleavage, yielding apo-carotenoids. Nevertheless, additional metabolic routes should be available, and some of the structures of those metabolites have only been anticipated. The identification of carotenoids and their metabolites is commonly performed by HPLC coupled to MS. The acquisition of MS data in different instrumental modes and hardware configurations allows the detailed structural description of the target compound. Indeed, the MS data of carotenoids and their metabolites available in literature could be collected in a database to automatically perform a screening of the theoretical MS data included in the database with the MS experimental values. This review describes the required fundamentals for a systematic pursuit of carotenoids and their metabolites. Highlights on the use of appropriate hyphenated HPLC-MS systems and the requirements for the identification are discussed, while the application of software tools to apply filtering rules and the implementation of post-processing workflow, which uses two orthogonal criteria for the identification from the mass spectra data, are described.
Description2 Tablas.-- 1 Figura
Publisher version (URL)http://dx.doi.org/10.1016/j.abb.2018.05.010
Identifiersdoi: 10.1016/j.abb.2018.05.010
e-issn: 1096-0384
issn: 0003-9861
Appears in Collections:(IG) Artículos
Files in This Item:
File Description SizeFormat 
PostP_2018_ABB_V650_P85.pdf248,22 kBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.