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Title

Detection of aluminum in lumbar spinal cord of sheep subcutaneously inoculated with aluminum-hydroxide containing products

AuthorsMiguel, Ricardo de; Asín, J.; Rodríguez-Largo, Ana; Molín, Jéssica; Echeverría, Irache; Andrés, Damián F. de ; Pérez, Marta M.; Blas, Ignacio de; Mold, Matthew; Reina, Ramsés ; Luján, Lluís
KeywordsAluminum hydroxide
Aluminum-based vaccines
Sheep
Ovine
Central nervous system
Issue DateMar-2020
PublisherElsevier BV
CitationJournal of Inorganic Biochemistry 204: 110871 (2020)
AbstractThe use of vaccines containing aluminum (Al) adjuvants is widespread in ovine production. Al adjuvants induce an effective immune-response but lead to the formation of post-vaccination granulomas from which Al can disseminate. This work aims to study the accumulation of Al in the central nervous system of sheep subcutaneously inoculated with Al-hydroxide containing products. Lumbar spinal cord and parietal lobe from 21 animals inoculated with 19 doses of Vaccine (n = 7), Adjuvant-only (n = 7) or phosphate-buffered saline as Control (n = 7) were analyzed with transversely heated graphite furnace atomic absorption spectroscopy and lumogallion staining for Al analytical measurements and Al tisular localization respectively. In the lumbar spinal cord, Al median content was higher in both the Adjuvant-only and Vaccine group (p = .001) compared with the Control group. Animals of the Adjuvant-only group showed the higher individual measurements in the lumbar spinal cord (14.36 μg/g and 7.83 μg/g). In the parietal lobe, Al median content tended to be higher in the Adjuvant-only group compared with Control group (p = .074). Except for three replicates of the Adjuvant-only group, Al content was always below 1 μg/g. In the lumbar spinal cord, lumogallion-reactive Al deposits were more abundant in the gray matter than in the white matter in both Vaccine (p = .034) and Adjuvant-only groups (p = .017) and Al deposits were mostly associated with glial-like cells (p = .042). In the parietal lobe, few Al deposits, which were sometimes related to blood vessels, were found. In sheep, Al-hydroxide adjuvants inoculated in the subcutaneous tissue selectively accumulate in the lumbar spinal cord.
Publisher version (URL)http://dx.doi.org/10.1016/j.jinorgbio.2019.110871
URIhttp://hdl.handle.net/10261/205970
Identifiersdoi: 10.1016/j.jinorgbio.2019.110871
issn: 1873-3344
issn: 0162-0134
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