Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/204323
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Cis-Acting Relaxases Guarantee Independent Mobilization of MOBQ4 Plasmids |
Autor: | Garcillán-Barcia, M. Pilar; Cuartas-Lanza, Raquel CSIC; Cuevas, Ana CSIC; Cruz, Fernando de la CSIC ORCID | Palabras clave: | Mobilizable plasmids Horizontal gene transfer MOBQ relaxase Cis-acting relaxase Plasmid coexistence Bacterial conjugation |
Fecha de publicación: | 8-nov-2019 | Editor: | Frontiers Media | Citación: | Frontiers in Microbiology 10: 2557 (2019) | Resumen: | Plasmids are key vehicles of horizontal gene transfer and contribute greatly to bacterial genome plasticity. In this work, we studied a group of plasmids from enterobacteria that encode phylogenetically related mobilization functions that populate the previously non-described MOBQ4 relaxase family. These plasmids encode two transfer genes: mobA coding for the MOBQ4 relaxase; and mobC, which is non-essential but enhances the plasmid mobilization frequency. The origin of transfer is located between these two divergently transcribed mob genes. We found that MPFI conjugative plasmids were the most efficient helpers for MOBQ4 conjugative dissemination among clinically relevant enterobacteria. While highly similar in their mobilization module, two sub-groups with unrelated replicons (Rep_3 and ColE2) can be distinguished in this plasmid family. These subgroups can stably coexist (are compatible) and transfer independently, despite origin-of-transfer cross-recognition by their relaxases. Specific discrimination among their highly similar oriT sequences is guaranteed by the preferential cis activity of the MOBQ4 relaxases. Such a strategy would be biologically relevant in a scenario of co-residence of non-divergent elements to favor self-dissemination. | Descripción: | © 2019 Garcillán-Barcia, Cuartas-Lanza, Cuevas and de la Cruz. | Versión del editor: | http://dx.doi.org/10.3389/fmicb.2019.02557 | URI: | http://hdl.handle.net/10261/204323 | DOI: | 10.3389/fmicb.2019.02557 | E-ISSN: | 1664-302X |
Aparece en las colecciones: | (IBBTEC) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Cis_Acting_Garcillan_Barcia_Art2019.pdf | 1,29 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
8
checked on 10-abr-2024
SCOPUSTM
Citations
11
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
11
checked on 27-feb-2024
Page view(s)
148
checked on 19-abr-2024
Download(s)
142
checked on 19-abr-2024