Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/20410
COMPARTIR / EXPORTAR:
logo share SHARE logo core CORE BASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE

Invitar a revisión por pares abierta
Título

Uptake and biological responses to nano-Fe versus soluble FeCl3 in excised mussel gills

AutorKádár, Enikö; Lowe, David M.; Solé, Montserrat CSIC ORCID ; Fisher, Andrew S.; Jha, Awadhesh N.; Readman, James W.; Hutchinson, Thomas H.
Palabras claveEngineered nanoparticles
Bivalves
Haemocycle
Oxidate stress
Neurotoxicity
Cytotoxicity
ENPs
Fecha de publicaciónene-2010
EditorSpringer Nature
CitaciónAnalytical and Bioanalytical Chemistry 396(2): 657-666 (2010)
ResumenNano-Fe particle uptake was experimentally examined in vitro using excised gills and blood cells of the edible blue mussel Mytilus sp. Whole gills were exposed to both Fe(2)O(3) nanoparticles and a solution of the hydrated FeCl(3) salt, for up to 12 h, and blood cells for 30 min. Equimolar Fe(+3) in the nano- and the soluble form was estimated under the assumption of dense spherical particles accommodating the same number of Fe(+3) as in the dissolved salt solution, namely: 1,000 microg L(-1) Fe(2)O(3) equivalent to 100 microg L(-1) FeCl(3).6H(2)O. Putative toxic impact of nano-Fe in gill epithelia and blood cells was assessed by an array of techniques including light- and electron microscopy, biomarkers for oxidative stress (lipid peroxidation levels), neurotoxic effects (acetylcholinesterase activity) and cytotoxicity (neutral red retention). Total and filtered fractions (20 and 200 nm, respectively) of Fe were analysed by ICP-OES. Our results provide evidence for the following: (1) much of both the soluble (95%) and the nano-Fe (90%) were removed from the water column within 12 h; (2) dissolved- and nano-Fe seemed to follow different routes of uptake within the gill epithelium; (3) both nano-Fe and soluble FeCl(3) caused similar impairment of lysosomal stability in circulating blood cells; (4) lipid peroxidation in gills exposed to the two distinct forms of Fe was increased, while acetylcholinesterase activity was unaffected. In these short-term in vitro studies, there appears to be little difference in toxic response between exposure to the Fe salt and the nano-Fe indicating that, in this case, the nanoparticles do not invoke special properties affecting biological function in gills. However, with the use of nano-Fe as a food additive, clearly longer-term in vivo
Descripción10 pages, 7 figures, 2 tables
Versión del editorhttps://doi.org/10.1007/s00216-009-3191-0
URIhttp://hdl.handle.net/10261/20410
DOI10.1007/s00216-009-3191-0
ISSN1618-2642
E-ISSN1618-2650
Aparece en las colecciones: (ICM) Artículos

Mostrar el registro completo

CORE Recommender

SCOPUSTM   
Citations

49
checked on 19-mar-2024

WEB OF SCIENCETM
Citations

45
checked on 27-feb-2024

Page view(s)

439
checked on 17-mar-2024

Google ScholarTM

Check

Altmetric

Altmetric


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.