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Título: | Pol5 is an essential ribosome biogenesis factor required for 60S ribosomal subunit maturation in Saccharomyces cerevisiae |
Autor: | Ramos-Sáenz, Ana CSIC; González-Álvarez, Daniel; Rodríguez-Galán, Olga CSIC ORCID; Rodríguez-Gil, Alfonso CSIC; Gaspar, Sonia G.; Villalobo, Eduardo CSIC ORCID; Dosil, Mercedes CSIC ORCID ; Cruz, Jesús de la CSIC ORCID | Palabras clave: | Ribosome biogenesis Pre-rRNA processing MYBBP1A Ribosomal proteins uL23 and eL27 Nucleolus |
Fecha de publicación: | 14-ago-2019 | Editor: | Cold Spring Harbor Laboratory Press RNA Society |
Citación: | RNA 25(11): 1561-1575 (2019) | Resumen: | In Saccharomyces cerevisiae, more than 250 trans-acting factors are involved in the maturation of 40S and 60S ribosomal subunits. The expression of most of these factors is transcriptionally coregulated to ensure correct ribosome production under a wide variety of environmental and intracellular conditions. Here, we identified the essential nucleolar Pol5 protein as a novel trans-acting factor required for the synthesis of 60S ribosomal subunits. Pol5 weakly and/or transiently associates with early to medium pre-60S ribosomal particles. Depletion of and temperature-sensitive mutations in Pol5 result in a deficiency of 60S ribosomal subunits and accumulation of half-mer polysomes. Both processing of 27SB pre-rRNA to mature 25S rRNA and release of pre-60S ribosomal particles from the nucle(ol)us to the cytoplasm are impaired in the Pol5-depleted strain. Moreover, we identified the genes encoding ribosomal proteins uL23 and eL27A as multicopy suppressors of the slow growth of a temperature-sensitive pol5 mutant. These results suggest that Pol5 could function in ensuring the correct folding of 25S rRNA domain III; thus, favoring the correct assembly of these two ribosomal proteins at their respective binding sites into medium pre-60S ribosomal particles. Pol5 is homologous to the human tumor suppressor Myb-binding protein 1A (MYBBP1A). However, expression of MYBBP1A failed to complement the lethal phenotype of a pol5 null mutant strain though interfered with 60S ribosomal subunit biogenesis. | Descripción: | © 2019 Ramos-Sáenz et al. | Versión del editor: | http://dx.doi.org/10.1261/rna.072116.119 | URI: | http://hdl.handle.net/10261/203702 | DOI: | 10.1261/rna.072116.119 | ISSN: | 1355-8382 | E-ISSN: | 1469-9001 |
Aparece en las colecciones: | (IBMCC) Artículos (IBIS) Artículos |
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Pol5_Ramos_Saez_Art2019.pdf | 5,67 MB | Adobe PDF | Visualizar/Abrir |
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