Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/203086COMPARTIR / EXPORTAR:
 SHARE
 CORE
BASE
|
|
| Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
| Título: | Disruption of the Caenorhabditis elegans Integrator complex triggers a non-conventional transcriptional mechanism beyond snRNA genes |
Autor: | Gómez-Orte, Eva; Sáenz-Narciso, Beatriz; Zheleva, Angelina CSIC ORCID; Ezcurra, Begoña; Toro, María de CSIC ORCID CVN; López, Rosario; Gastaca, Irene; Nilsen, Hilde; Sacristán, María P. CSIC ORCID CVN ; Schnabel, Ralf; Cabello, Juan CSIC ORCID | Fecha de publicación: | 26-feb-2019 | Editor: | Public Library of Science | Citación: | PLoS Genetics 15(2): e1007981 (2019) | Resumen: | Gene expression is generally regulated by recruitment of transcription factors and RNA polymerase II (RNAP II) to specific sequences in the gene promoter region. The Integrator complex mediates processing of small nuclear RNAs (snRNAs) as well as the initiation and release of paused RNAP II at specific genes in response to growth factors. Here we show that in C. elegans, disruption of the Integrator complex leads to transcription of genes located downstream of the snRNA loci via a non-conventional transcription mechanism based on the lack of processing of the snRNAs. RNAP II read-through generates long chimeric RNAs containing snRNA, the intergenic region and the mature mRNA of the downstream gene located in sense. These chimeric sn-mRNAs remain as untranslated long non-coding RNAs, in the case of U1- and U2-derived sn-mRNAs, but can be translated to proteins in the case of SL-derived sn-mRNAs. The transcriptional effect caused by disruption of the Integrator complex is not restricted to genes located downstream of the snRNA loci but also affects key regulators of signal transduction such as kinases and phosphatases. Our findings highlight that these transcriptional alterations may be behind the correlation between mutations in the Integrator complex and tumor transformation. | Descripción: | © 2019 Gómez-Orte et al. | Versión del editor: | http://dx.doi.org/10.1371/journal.pgen.1007981 | URI: | http://hdl.handle.net/10261/203086 | DOI: | 10.1371/journal.pgen.1007981 | ISSN: | 1553-7390 | E-ISSN: | 1553-7404 |
| Aparece en las colecciones: | (IBMCC) Artículos |
Ficheros en este ítem:
| Fichero | Descripción | Tamaño | Formato | |
|---|---|---|---|---|
| Disruption_Gomez_Orte_Art2019.pdf | 4 MB | Adobe PDF |  Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
20
checked on 27-mar-2024
SCOPUSTM
Citations
25
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
24
checked on 22-feb-2024
Page view(s)
157
checked on 18-abr-2024
Download(s)
102
checked on 18-abr-2024
