English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/20182
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

An oligo-based microarray offers novel transcriptomic approaches for the analysis of pathogen resistance and fruit quality traits in melon (Cucumis melo L.)

AuthorsMascarell-Creus, Albert; Cañizares, Joaquín; Vilarrasa-Blasi, Josep ; Mora-García, Santiago; Blanca, José M.; González-Ibeas, Daniel ; Saladié, Montserrat ; Roig, Cristina; Deleu, Wim; Picó-Silvent, Belén; López-Bigas, Nuria; Aranda, Miguel A. ; García-Mas, Jordi ; Nuez, Fernando; Puigdomènech, Pere ; Caño-Delgado, Ana I.
Issue DateOct-2009
PublisherBioMed Central
CitationBMC Genomics 10(467): (2009)
AbstractBackground: Melon (Cucumis melo) is a horticultural specie of significant nutritional value, which belongs to the Cucurbitaceae family, whose economic importance is second only to the Solanaceae. Its small genome of approx. 450 Mb coupled to the high genetic diversity has prompted the development of genetic tools in the last decade. However, the unprecedented existence of a transcriptomic approaches in melon, highlight the importance of designing new tools for high-throughput analysis of gene expression.
Results: We report the construction of an oligo-based microarray using a total of 17,510 unigenes derived from 33,418 high-quality melon ESTs. This chip is particularly enriched with genes that are expressed in fruit and during interaction with pathogens. Hybridizations for three independent experiments allowed the characterization of global gene expression profiles during fruit ripening, as well as in response to viral and fungal infections in plant cotyledons and roots, respectively. Microarray construction, statistical analyses and validation together with functional-enrichment analysis are presented in this study.
Conclusion: The platform validation and enrichment analyses shown in our study indicate that this oligo-based microarray is amenable for future genetic and functional genomic studies of a wide range of experimental conditions in melon.
Description15 pages, 4 figures, 3 tables, 1 appendix, 3 additional files.
Publisher version (URL)http://dx.doi.org/10.1186/1471-2164-10-467
URIhttp://hdl.handle.net/10261/20182
DOI10.1186/1471-2164-10-467
ISSN1471-2164
Appears in Collections:(CEBAS) Artículos
(CRAG) Artículos
Files in This Item:
File Description SizeFormat 
1471-2164-10-467.pdf2,77 MBAdobe PDFThumbnail
View/Open
Show full item record
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.